大鼠脾移植诱导特异性免疫耐受效果

目的 比较异位脾移植和原位脾移植诱导特异性免疫耐受的效果.方法 建立大鼠异位脾移植和原位脾移植模型6周后,二期行同源心脏移植,比较移植心脏的存活时间和移植5d后的排斥反应强度.以单纯心脏移植组和心脏移植+环孢菌素组作为对照组.结果 移植心脏存活时间:心脏移植+环孢菌素组＞原位脾移植组＞异位脾移植组＞单纯心脏移植组(P＜0.05).术后第7天排斥反应强度和混合淋巴细胞反应强度测定:心脏移植+环孢菌素组＜原位脾移植组＜异位脾移植组＜单纯心脏移植组(P＜0.05).结论 由于脾脏的生理功能与门静脉系统的回流特点有关,较之于异位脾移植,原位脾移植能更为有效地诱导受体特异性免疫耐受状态.

Specific immunological tolerance induced by spleen transplantation in rats

Objective To compare the efficiency of vaseularized spleen orthotopic allotransplantation(VSOT)with vascularized spleen heterotopic allotransplantation(VSHT)in inducing immunological tolerance.Methods Secondary heart transplantation was performed 6 weeks after the establishment of spleen transplantation model and heart transplantation with or without CsA treatment served as negative or positive control.Survival time of heart transplants,intensity of rejection and mixed lymphatic reaction were detected in each group 5 days after transplantation.Results Heart transplants in each group survived longest in CsA group,followed by those in VSOT group,VSHT group and heart transplantation group.Intensity of rejeetion and mixed lymphatic reaction were strongest in heart transplantation group,followed by those in VSHT group,VSOT group,and CsA group.Conclusion VSOT is much more efficient than VSHT in inducing immunological tolerance.