| 急性髓系白血病ID4基因甲基化研究 |
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| 引用本文： | Xu RR,Liu F,Cui X,Zhang XW,Wang Y. 急性髓系白血病ID4基因甲基化研究[J]. 中国实验血液学杂志, 2011, 19(3): 582-584 |
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| 作者姓名： | Xu RR Liu F Cui X Zhang XW Wang Y |
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| 作者单位： | 山东中医药大学附属医院血液科,山东济南,250011 |
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| 摘 要： | 本研究旨在探讨急性髓系白血病(AML)患者中ID4基因甲基化状态。采用甲基化特异性聚合酶链反应(M S-PCR)对46例不同亚型、不同病期的AML患者骨髓细胞进行ID4基因启动子区甲基化状况检测,并以10例缺铁性贫血患者骨髓作为对照。结果表明,ID4基因在对照组患者骨髓中呈完全性非甲基化状态,在46例AML患者中有39例患者出现ID4基因甲基化(阳性率为84.8%),其中各AML亚型M1、M2、M3、M4、M5、M6患者ID4基因甲基化阳性率分别为4/4、9/12、8/8、7/9、9/11、2/2。初治,完全缓解的AML患者ID4基因甲基化阳性率分别为27/30、7/11,另有5例复发难治患者均检测出ID4基因甲基化,与对照组相比,差异均有统计学意义(p<0.01)。结论 :与对照组患者相比,AML不同亚型、不同病期患者ID4基因发生了不同程度的甲基化改变,这表明ID4基因甲基化可能是AML发生发展过程中的一个早期分子事件。
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| 关 键 词： | ID4基因 急性髓系白血病 DNA甲基化 |
ID4 promoter methylation in acute myeloid leukemia |
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| Xu Rui-Rong,Liu Fei,Cui Xing,Zhang Xue-Wei,Wang Yan. ID4 promoter methylation in acute myeloid leukemia[J]. Journal of experimental hematology, 2011, 19(3): 582-584 |
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| Authors: | Xu Rui-Rong Liu Fei Cui Xing Zhang Xue-Wei Wang Yan |
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| Affiliation: | XU Rui-Rong,LIU Fei,CUI Xing,ZHANG Xue-Wei,WANG Yan Department of Hematology,Hospital Affliated to Shandong University of Traditional Chinese Medicine,Jinan 250011,Shandong Province,China |
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| Abstract: | Objective of this study was to investigate the ID4 gene methylation status in patients with acute myeloid leukemia(AML).Methylation-specific PCR(MS-PCR) was used to detect the promoter methylation status of ID4 gene in bone marrow samples from 46 AML patients with different subtypes and stage of disease and from 10 patients with iron deficiency anemia(IDA) as a control.The results showed that ID4 gene in bone marrow of IDA patients was completely non-methylated,while ID4 gene methylation was found in 39 out... |
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| Keywords: | id4 gene acute myeloid leukemia DNA methylation |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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