细胞外信号调节激酶在糖尿病大鼠肺病变中的作用

目的　观测糖尿病大鼠肺组织的病理改变及蛋白激酶C、胞外调节蛋白激酶活性的变化 ,探讨细胞信号传导系统在糖尿病大鼠肺病变中的作用。方法　制作糖尿病大鼠模型 ,4w后应用透射电镜观察大鼠肺组织病理改变 ,采用改良的Takay法测定蛋白激酶C活性 ,同位素法及蛋白质免疫印迹分析方法检测胞外调节蛋白激酶在糖尿病大鼠肺组织表达的变化。结果　糖尿病大鼠 4w肺组织病理改变为毛细血管基底膜及Ⅱ型肺泡上皮细胞基底膜不同程度增厚 ,肺间质胶原成份增多 ,蛋白激酶C、胞外调节蛋白激酶在肺组织活性增强。结论　链脲菌素糖尿病大鼠肺组织高糖环境下细胞内信号传导系统被激活 ,可能参与了糖尿病肺部并发症的发生和发展

The alteration of extracellular signal-regulated kinase in lung tissues in diabetic rats

Objective To observe the pathologic changes and the activities alteration of protein kinase C (PKC) and extracellular signal regulated kinase (ERK) in lung tissues in diabetic rats, and to investigate the role of signal pathways in pulmonary variety in diabetic rats.Methods Diabetic model rats were induced by injected streptozotozin. After 4 weeks, we observed the pathologic changes in lungs, tested the activities of PKC and ERK by isotope in lungs of model rats, determined the expression of ERK using Western blot.Results After STZ administration for 4 weeks, we observed thickened pulmonary capillary basal lamina and increasing number of fibre in diabetes mellitus rats. The activities PKC and ERK were found increased.Conclusions Hyperglycemia protein kinase C ERK signal pathways might be involved in the pathogenesis in lung tissues in diabetic rats.