褪黑素对大鼠脑损伤的保护作用及机制研究

目的:探讨褪黑素(MT)对大鼠脑损伤的保护作用及其机制。方法:采用自由落体撞击伤方法制作创伤性脑损伤模型,致伤后连续给予褪黑素,测定脑损伤后12h、24h、48h、72h大鼠脑组织谷胱甘肽过氧化物酶(GSH-PX)活性、丙二醛(MDA)和钙离子(Ca2+)含量并观察脑微细结构的变化。结果:创伤性脑损伤后脑组织GSH-PX活性降低,MDA及Ca2+含量升高,褪黑素能部分反转这种变化,脑水肿减轻。结论:褪黑素对创伤性脑损伤有保护作用,其机制可能与保护GSH-PX活性,减少脂质过氧化有关。

Protective effects and mechanisms of melatonin on brain contusion in rats

Objective：To investigate the protective effects and mechanisms of melatonin on brain contusion . Methods：72 Sprague-Dawley rats were divided into three groups. Traumatic braininjury has been developed through Feeney＇s method, melatonin was given after being injuried and mensurated the activities of GSH-PX and the contents of MDA and Ca^2＋ in rat brain serum afterl2h, 24h,48h,and 72h of brain contusion. The morphological change was observed at the injured area by LM. Results： The activities of GSH-PX reduced in rat brain serum following traumatic brain injury, but contents of MDA and Ca^2＋ increased. Melatonin treatment partly reversed these changes and reduced the brain edema. The effect of melatonin was also confirmed histopathologically. Conclusion：Melatonin is neuroprotective in traumatic brain injury, its probable mechanism is related to protect the activities of GSH-PX. Melatonin may be a valuable therapeutic agent in the treatment of brain contusion.