db／db2型糖尿病小鼠的肠道、门脉血中LPS水平与血糖的相关性分析

目的：探讨LPS水平与2型糖尿病血糖相关性。方法：采用db／db2型糖尿病小鼠db／db组和对照组db／m，收集门脉血、结肠组织并取匀浆液，用酶联免疫法（ELISA）测定肠道、门脉血中脂多糖的水平；用血糖仪测血糖值。结果：db／db模型组与db／m组相比，肠道LPS水平高（P〈0．05），门脉血中LPS水平高（P〈0．05），肠道比门脉血中LPS水平高（P〈0．05），FPG是高的（P〈0．05），结肠、门脉血中LPS分别与血糖成正相关（r=0．72，P〈0．01；r=0．71，P〈0．01）。结论：LPS水平与2型糖尿病的血糖有关，可为糖尿病的机制的研究提供有力的依据。

Correlation between The LPS level in intestines and portal blood and FPG of db / db type 2 diabetic mellitus mice

Objective： To detecte correlation between The LPS level in intestines and portal blood and FPG of db/db type 2 diabetic mellitus mice. Methods ： To select type 2 diabetic mellitus mice db / db group and control db/m group, the collection of portal blood and take colon tissue homogenate, LPS level in intestinal and portal blood were measured by enzyme - linked immunosorbent assay （ELISA） ; FPG were measured by Blood glucose meter Results： Compared with db/m group, db / db model group were that LPS level in intestinal and in portal blood were increased （ P 〈 0. 05 ）, and the LPS level in intestinal than portal blood is higher （ P 〈 0. 05 ）, FPG is inrcesed （ P 〈 0. 05 ）, LPS in colon and portal blood and blood glucose was positively related （ r = 0. 72, P 〈 0. 1 ; r = 0.71, P 〈0. 01 ）. Conclusion： LPS level is associated with blood glucose of type 2 diabetes, can provide a strong basis for the mechanism of diabetes research.