弓形虫RH株在实验感染小鼠体内分布的研究

以间接免疫酶法观察弓形虫感染时的急性期病变特点及虫体在主体脏器的动态分布，以期为弓形虫病的病理诊断提供依据，并对弓形虫致病机理加深理解。方法用弓形虫ＲＨ株速殖子１０＾３个经腹腔感染昆明小鼠，于感染后第２、４、６和８ｄ，取肝、脾、肺和脑进行间接免疫酶染色。

STUDY ON THE DISTRIBUTION OF RH STRAIN TOXOPLASMA GONDII IN EXPERIMENTAL INFECTED MICE

Aim In order to observe the pathological features and the dynamic distribution of RH strain T.gondii in main organs of infected mice, using indirect immunoenzymatic technique. To provide pathological diagonsising reference of toxoplasmosis and increase to understand the pathologensis of Toxoplasmosis. Methods Mice were infected intraperitoneally with 10 3 tachyzoites of T.gondii RH strain,and the parasites were detected using indirect immunoenzymatic technique in the liver, spleen, lungs and brain at 2,4,6 and 8 days postinfection. Results The liver was the first organ parasitized at D2, followed by spleen and lungs at D4, the brain at D8. At the early phase of infection, parasites were found on the edge of the liver and spleen. A few parasites were detected within the liver, spleen and lungs with time being. But parasites increased progressively and distributed well during the whole phase. The brain was the last organ to be parasitized. Parasites multiplicated rapidly so that the mice were seriously ill and died. Conclusions The indirect immunoenzymatic technique can demonstrate tachyzoites and Toxoplasma antigen clearly in infected mice during acute stage. Many organs were infected such as liver,spleen,lungs and brain. The results suggest that the organs in the peritoneal cavity were infected directly by tachyzoites as IP infection, then the parasites disseminate through a blood way, and in the end, tachyzoites cross the blood-brain barries to reach the brain.