血清HBV-DNA定量PCR检测对干扰素治疗的指导意义

目的 探讨有限稀释定量ＰＣＲ检测血清ＨＢＶ－ＤＮＡ对干扰素抗病毒治疗的指导意义。方法 利用有限稀释定量ＰＣＲ技术分别检测３８例ＨＢｓＡｇ、ＨＢｅＡｇ双阳性患者α－２ｂ干扰素治疗前后血清ＨＢＶ－ＤＮＡ含量。结果 ３８例患者干扰素治疗１２周ＨＢＶ－ＤＮＡ阴转７例（１８．４％）；治疗２４周阴转１１例（２８．９％）。其中病毒滴度〉２５０ｐｇ／ｍＬ者阴转１１．１％（１／９）；病毒滴度２５ｆｇ￣２５０ｐｇ／ｍ

Directive value of quantitative PCR of serum HBV-DNA for interferon treatment in patients with chronic hepatitis B

To observe the directive value of limited dilution quantitative PCR determination of serum HBV-DNA on the efficacy of interferon anti-virus in patients with chronic hepatitis B. Method HBV-DNA concenhations were quantitatively determined before and after interferon treatment in 38 patients with HBsAg( ) and HBeAg( ) by the footed dilution PCR. Results Among 38 patients with interferon treatment for 12 and 24 weeks, HBV-DNA was negative in 7 cases (18 .4% ) and 11 cases (28. 9% ) respectively. There was only 1 case (11 .5% ) negative in the group of virus titer > 250 pg/ mL (9 cases), but 4 and 6 cases (25.0% and 46.2%) negative in the groupe of virus titer 25 fg~ 250 pg/mL and < 25fg/ mL (P < 0.01). Conclusions HBV-DNA concentration could be detendned quantitatively by limited dilution PCR, which showed some directive effect on interferon anti-virus in patients with chronic hepatitis B. The patients whose virus titer 250 pg/mL and treatment for 24 weeks with interferon would receive better effect.