哮喘小鼠CD4+T细胞中microRNA-31和FOXP3mRNA的表达及相互关系的研究

目的 初步探讨哮喘CD4+T细胞中microRNA-31和FOXP3的表达水平、两者的相关性及激素对它们的影响.方法 以卵蛋白(OVA)致敏激发建立哮喘小鼠模型.将15只BALB/c小鼠分为正常对照组、OVA组和地塞米松组,EHSA检测小鼠支气管肺泡灌洗液(BALF)中IL-4和IFN-γ水平；实时荧光定量PCR检测小...

Expression and relationship of microRNA-31 and FOXP3 mRNAs in CD4~+ T cells from a mouse asthma model

Objective To determine the expression levels of microRNA-31 and FOXP3 mRNAs in the spleen CD4+ T cells from asthma mice,and identify their relationship.Methods A total of 15 Balb/c mice were randomly divided into 3 groups,normal control group,model group,and dexamethasone treatment group.The murine asthma model was sensitized and challenged by ovalbumin(OVA).The inflammatory cytokines IL-4 and IFN-γ in the bronchoalveolar lavage fluid(BALF) in the 3 groups were measured by ELISA.Expression of microRNA-31 and FOXP3 mRNAs in spleen CD4+ T cells from the 3 groups were detected by quantitative real time-PCR assay.The relationship of the expression were also analyzed.Results The BALF level of IL-4 was increased in the model group(228.29±66.18 pg/ml) compared with the normal control group(66.63±17.33 pg/ml,P<0.05),but no significant difference was found in its level between the dexamethasone treatment group(120.84±32.29) and normal control(P>0.05).There was no significant difference in levels of IFN-γ among the three groups(P>0.05).The expression of FOXP3 mRNA in the spleen CD4+ T cells in the model group was 0.10 times as great as in the normal control group(P<0.05),and that in the dexamethasone treatment group was 3.75 times as great as in the model group(P<0.05).However,there was no significant difference in FOXP3 mRNA between the normal control and the dexamethasone treatment group(P>0.05).The expressions of microRNA-31 mRNA in the splenn CD4+ T lymphocytes in the model group were 4.79 times as great as in the normal control(P<0.05),and that in the dexamethasone treatment group were 4.85 times as great as in the normal control(P<0.05).There was no significant difference in microRNA-31 expression between the model group and the dexamethasone treatment group(P>0.05).Pearson correlation coefficient between microRNA-31 and FOXP3 mRNA was-0.609(P<0.05).Conclusion There is a negative linear correlation between microRNA-31 and FOXP3 mRNA in asthma,and microRNA-31 and dexamethasone may affect the expression of FOXP3 mRNA via different pathways.