壳聚糖-胶原支架与人牙周膜细胞复合培养的实验研究

目的 对不同比例的壳聚糖-胶原（chi—col）进行细胞相容性和细胞毒性的研究，以初步探讨其应用于牙周组织工程的可行性。方法 将体外培养的人牙周膜细胞（PDLCs）接种于不同比例的chi—col上复合培养，采用细胞计数评价PDLCs在clli—col上的附着、生长情况，并通过MTF测试法和碱性磷酸酶（ALP）活性检测法研究chi—col浸提液对PDLCs增殖和功能表达的影响。结果 人PDLCs在chi—col上形成良好贴附并增殖，而PDLCs在不同浓度的材料浸提液中的生长、增殖及ALP活性与对照组相比无统计学意义。结论 clli—col具有良好的三维空间结构和细胞相容性，且无细胞毒性，有望成为牙周组织工程的支架材料。

The Study of Human Periodontal Ligament Cells on Chitosan- collagen Applying

Objective The cytocompatibility and cytotoxicity of chitosan-collagen(chi-col) were investigated to evaluate the feasibility of chi-col as the scaffold material of periodontal tissue engineering.Methods Human periodontal ligament cells(PDLCs) cultured in vitro were collected and seeded on three-dimensional framework of chi-col.The cell growth in the scaffolds was observed by cell counting.And the influences of the chi-col extracts on cell proliferation and differentiation were observed by MTT assay and Alkaline phosphatase(ALP) activity assay.Results Cells adhered and proliferated on the three-dimensional framework of chi-col showed the porous structure of chi-col and the eugonic growth of cells in the scaffolds.The different concentrations of chi-col extracts had no influences on the cell proliferation and differentiation.Conclusions Provided with the porous structure and satisfactory cytocompatibility and without any cytotoxicity,chi-col is valued to further study as the scaffolds of periodontal tissue engineering.