| HBV—DNA荧光定量PCR检测下限的确立 |
| |
| 引用本文： | 梁金明,陈亚珍,李艳. HBV—DNA荧光定量PCR检测下限的确立[J]. 分子诊断与治疗杂志, 2010, 2(4): 257-259 |
| |
| 作者姓名： | 梁金明 陈亚珍 李艳 |
| |
| 作者单位： | 广东省湛江中心人民医院检验科,广东,湛江,524037 |
| |
| 摘 要： | 目的 确立本实验室荧光定量PCR检测HBV-DNA项目的检测下限.方法 分别测定原倍HBV-DNA质控血清及经100、200倍稀释后HBV-DNA含量.结果 原倍质控血清测定结果的均值为3.63×104 IU/mL（4.56）,在给定靶值范围2.14×104～2.14×105 IU/mL（4.33～5.33）的低值附近;100倍稀释后的检测结果的均值为3.32 × 102 IU/mL（2.52）,在试剂盒最低检测下限（500 IU/mL）以下,且100份标本能100%定量检测.结论 本实验室设备及所用的试剂、方法对HBV-DNA〉500 IU/mL的标本完全有能力定量检测;检测下限为500 IU/mL是符合要求的.
|
| 关 键 词： | 荧光定量聚合酶链反应 HBV-DNA 检测下限 |
Determination of detection limit of HBV-DNA by real-time fluorescence quantitative PCR |
| |
| LIANG Jinming,CHEN Yazhen,LI Yan. Determination of detection limit of HBV-DNA by real-time fluorescence quantitative PCR[J]. Journal of Molecular Diagnosis and Therapy, 2010, 2(4): 257-259 |
| |
| Authors: | LIANG Jinming CHEN Yazhen LI Yan |
| |
| Affiliation: | (Department of Laboratory, Central People＇s Hospital of Zhanjiang, Guangdong, Zhanjiang 524037, China) |
| |
| Abstract: | Objective To determine the lower limit of HBV-DNA by real-time fluorescence quantitative PCR for our laboratory. Methods The quality control serum for hepatitis B virus DNA and 100- fold, 200-fold dilution quality control serum of HBV-DNA were tested. Results The HBV-DNA quality control serum was 3.63 x 104 IU/mL（4.56）.The 100-fold dilution samples was 3.32× 10^2 IU/mL（2.52）, within the detection limit of the kit （500 IU/mL）. Conclusion With the reagents of real-time fluorescence quantitative PCR, the detection limit（500 IU/mL） meet the demand. |
| |
| Keywords: | FQ-PCR HBV-DNA Limit of detection |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
