循经电针刺激可激活穴区皮下感觉传入神经-CB-HRP示踪研究

本实验是将1％CB－HRP5μl分别注入大鼠一侧“足三里”或“乳中”穴区皮下,追踪不同节段脊神经节内被标记细胞,观察穴区皮下感觉传入神经的节段性分布及循经电针刺激对此节段性分布的影响,结果发现：“足三里”穴区皮下感觉神经末梢主要投射至L4、L5两脊神经节,“乳中”穴区皮下感觉神经主要投射至T4、T5、T6脊神经节;循经电针刺激可激活“足三里”或“乳中”穴区皮下感觉神经末梢,使其摄取HRP能力增强,因而同一脊神经节阳性标记细胞数增加,不同脊神经节出现阳性标记细胞的节段扩大。以上结果为循经感传的跨神经节段性传入提供了一些形态学依据。

Electroacpuncture along Meridians Activating Subcutaneous Primary Afferents in Acupoints-CB-HRP Tracing Study

The experiment was performed to observe the segmental distribution of sensory afferent nerves in acupoints and the influence of electroacupuncture(EA) along meridians on it. 1% CB-HRP solution (5μl) was injected subcutaneously at unilateral"Zusanli"or"Ruzhong"acupoint of rats. The HRP labeled cells were counted in the related spinal dorsal root ganglia(DRG). The results were as follows: the sensory nerve terminals of "Zusanli"acupoint mainly projected to the neurons of L4 and L5 DRG and that of "Ruzhong" acupoint proected to the neurons of T4、T5 and T6 DRG;sensory nerve terminals of "Zusanli" or "Ruzhong"acupoint were activated by the EA stimulation along meridians, their ability of uptaking HRP was enhanced, so the HRP labeled cells increased in the related DRG, the HRP labeled DRG segments spread. This study provides a new morphological evidence for the mechanism's explanation of propagated sensation along the meridians