肺癌患者外周血和骨髓中MUC1 mRNA表达的临床意义

目的:检测非小细胞肺癌(non smal celllungcancer,NSCLC)外周血和骨髓中粘蛋 白1(mucin1,MUC1)mRNA的表达。方法:应用 RT PCR技术,对31例肺癌患者、10例肺良性病 变患者和8名健康人外周血及骨髓中MUC1基 因mRNA表达进行检测。结果:31例肺癌患者 中10例检测到外周血,7例检测到骨髓中有 MUC1mRNA表达,10例肺良性病变患者和8名 健康人中未检测到MUC1mRNA表达。肺癌患 者外周血及骨髓中MUC1mRNA表达分别为 32.3%(10/31)和22.6%(7/31),两者之间存在 正相关关系,P<0.05;外周血和骨髓中MUC1 mRNA的表达与肺癌组织学类型、细胞分化程度 及pTNM分期均密切相关,P<0.05。结论:肺癌 患者外周血和骨髓中存在常规方法检测不出的 肿瘤细胞;应用巢式RT PCR法可检测到肺癌患 者外周血和骨髓中MUC1mRNA表达,可为制 定治疗方案和评估预后提供重要参考依据。

Clinical significance of MUC1 mRNA expression in peripheral blood and bone marrow in patients with lung cancer

OBJECTIVE: To detect MUC1 mRNA expression in peripheral blood and bone marrow in patients with non-small cell lung cancer (NSCLC), and to evaluate the clinical significance of MUC1 mRNA expression. METHODS: Nested RT-PCR was used to detect MUC1 mRNA expression in peripheral blood and bone marrow in 31 patients with NSCLC. RESULTS:Positive rates of MUC1 mRNA were 32.3%(10/31) in peripheral blood and 22.6%(7/31) in bone marrow in patients with NSCLC, and highly positive correlation between MUC1 expression in peripheral blood and in bone marrow was found. MUC1 expression was not found in 10 patients with benign pulmonary lesion and 8 normal adults. The MUC1 mRNA expressions in peripheral blood and in bone marrow were closely related to the pathological classification, cell differentiation of cancer and P-TNM stage of the disease,P<0.05. CONCLUSIONS:MUC1 mRNA may be a valuable marker to detect DTC in patients with lung cancer. It can also provide important clinical information for the evaluation of prognosis and the selection of appropriate treatment strategies.