HPLC法测定阿苯达唑片的含量与有关物质

目的：建立高效液相色谱法测定阿苯达唑片的含量与有关物质.方法：采用Agilent XDB-C18色谱柱（250 mm＆#215;4.6 mm,5 μm）,流动相：0.015 mo l·L-1磷酸二氢铵-甲醇（ 30：70）,流速0.8 ml·min-1,检测波长295 nm,柱温30℃,进样量20 μl.结果：阿苯达唑在6.25～ 125.00 μg·ml-1范围内线性关系良好（r=0.999 9）,平均回收率为100.2%（RSD=0.6%,n=9）,有关物质各杂质峰与阿苯达唑峰的分离度良好,检测限为2 ng.结论：本方法简便、快速、准确,专属性好,可用于阿苯达唑片含量和有关物质的测定.

Determination of Content and Related Substances of Albendazole Tablets by HPLC

Objective ： To establish an HPLC method for determination of the content and related substances of albendazole tablets. Methods： An Agilent XDB-C18 column（250 mm ×4.6 mm, 5 μm） was adopted for the HPLC analysis, the mobile phase was 0.015 mo 1 · L - l ammonium phosphate monobasic-methanol （ 30 ： 70 ） with a flow rate of 0.8 ml ·min - 1 , the detection wavelength was 295nm, the column temperature was at 30℃ and the injection volume was 20 μ1. Results： The linear range of albendazole was 6.25- 125.00 μg · ml-1 （ r = 0.9999 ）. The average recovery was 100.2% （ RSD = 0.6%, n = 9）. Albendazole and the related substance could be well separated. The detection limit was 2ng. Conclusion ： The method is simple, rapid and accurate, and suitable for the de- termination of albendazole tablets and the related substances.