王不留行总皂苷提取工艺优选

目的：优选王不留行总皂苷的提取工艺。方法：以总皂苷质量分数和干粉得率为综合评价指标，采用正交试验考察料液比、乙醇体积分数、超声时间、超声功率对超声波提取工艺的影响，考察提取时间、乙醇体积分数、料液比和提取次数对乙醇回流提取工艺的影响。结果：最佳超声提取工艺为加15倍量70%乙醇在640 W功率下超声提取2次，每次40 min；干粉得率7.34%，总皂苷质量分数10.92%。最佳乙醇回流提取工艺为加14倍量60%乙醇在微沸状态下提取2次，每次2 h；干粉得率7.17%，总皂苷质量分数9.01%。结论：王不留行总皂苷超声波辅助法的提取效果优于乙醇回流法，且工艺稳定可靠。

Optimization of Extraction Process for Total Saponins from Vaccariae Semen

Objective: To optimize extraction technology of total saponins from Vaccariae Semen. Method: With dry powder yield and the mass fraction of total saponins as comprehensive evaluation index,orthogonal tests were adopted to optimize ultrasonic and ethanol reflux extraction processes by taking extraction time,solid-liquid ratio and ultrasonic power,ethanol concentration and extraction times as factors. Result: Optimum ultrasonic extraction conditions were as follows:extracted twice with 15 times the amount of 70% ethanol under 640 W,20 minutes per time;Dry powder yield and the mass fraction of total saponins were 7.34% and 10.92%,respectively.Optimum ethanol reflux extraction technology was as following:extracted twice with 14 times the amount of 60% ethanol under micro-boiling state,2 h per time;Dry powder yield and the mass fraction of total saponins were 7.17% and 9.01%,respectively. Conclusion: Ultrasonic assisted extraction method was better than ethanol reflux extraction method for total saponins from Vaccariae Semen,which were stable and reliable.