沈阳地区不同人群HCV感染及HCV基因型分布研究

应用酶联免疫检测技术 ,检测沈阳地区 390 2例不同人群血清的抗 - HCV,并应用引物特异 PCR法对其中 10 0例血清标本进行 HCV基因分型。结果 :正常人群抗 - HCV阳性率为 0 .4 2 %～ 1.66% ;输血后及散发性肝炎 (除外 HAV、HBV、EBV、CMV感染 )、乙型肝炎、肝炎后肝硬化 (除外 HBV感染 )、原发性肝癌等组抗 - HCV阳性率显著高于无输血史的非肝病病人组 ( P<0 .0 1) ;10 0例血清标本中 HCV- 型感染占 58%、HCV- 型 2 7%、 / 混合型 14%、未分型 1% ,正常人群HCV感染者中 HCV基因型以 型为主 ( 80 % ) ,丙型肝炎后肝硬化病人 HCV基因型以 型为主( 91.7% )。提示 :各类肝病病人是 HCV感染的高危人群 ;沈阳地区 HCV感染以 型为主 ,其次为 型及 / 混合型 ,HCV基因型可能与丙型肝炎病情轻重有关。

Study on HCV Infection and the Distribution of HCV Genotypes in Different Populations in Shenyang Area

serum samples in different populations were tested for anti- HCV by EL ISA. Among10 0 samples of them,genotypes of HCV were detected by PCR with type- specific primers. The results showed:a) anti- HCV positive rates in general populations were0 .4 2 % - 1.66% ;b) anti- HCV positive rates in the group of post- transfusion hepatitis and sporadic hepatitis( excluding HAV,HBV,EBV,CMV infection) ,hepatitis B post hepatitis cirrhosis( excluding HBV infection) and primary hepatocarcinoma were significantly higher than the group of patients without liver diseases or the group of patients without transfusion( P<0 .0 1) ;c) in HCV genotyping,HCV- , HCV- , / mixed type were58% ,2 7% ,14% respectively.The rate of infection with HCV- was80 % in normal population but91. 7% with type HCV in the group of patients with cirrhosis.These results suggested:( 1) patients with different liver diseases were under high risk of HCV infection;( 2 ) type HCV- was predominant followed by HCV- as well as / mixed in HCV genotype distribution of Shenyang area.Different genotypes of HCV might be related to the severity of hepatitis C.