水蛭素对凝血酶诱导的血管平滑肌细胞增殖的影响

为研究重组水蛭素对凝血酶诱导的兔胸主动脉血管平滑肌细胞增殖的影响及其作用机制.选用第2～5代培养的血管平滑肌细胞,在与凝血酶(4.0 ku/L)共同孵育的条件下,分别给予水蛭素(6.0 ku/L)和肝素(6.O ku/L)进行干预.通过四唑盐比色实验检测细胞生长活性,流式细胞术检测细胞周期,免疫组织化学检测血小板生长因子和增殖细胞核抗原在血管平滑肌细胞中的表达.结果发现,重组水蛭素能明显抑制凝血酶诱导的平滑肌细胞增殖,其抑制作用可能与减低了血管平滑肌细胞对血小板生长因子和增殖细胞核抗原的表达有关.

Influence of Hirudin on Thrombin-induced Proliferation of Vascular Smooth Muscle Cells

Aim To study the influence of hirudin on thrombin-induced proliferation of vascular smooth muscle cells (VSMC) and its regulated mechanism in vitro. Methods VSMCs were isolated from the thoracic aorta of rabbits and cultured 2 - 5 passages were incubated with thrombin (4.0 ku/L), while hirudin (6.0 ku/L) and heparin (6.0 ku/L) were added except for control group. The effect of hirudin on growth of VSMC was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet-razohum bromide (MTT) assay and flow cytometry. The expression of platelet-drived growth factor (PDGF) and proliferating cell nuclear antigen (PCNA) in VSMCs was evaluated by immunohistochemistry technique and computer image analysis system. Results Hirudin remarkedly inhibited both the thrombin-induced proliferation and the expression of PDGF and PCNA in VSMC (P < 0.05, P < 0.01). Conclusion Hirudin is able to significantly influence the proliferative activity of VSMCs, whichmay be carried out through inhibition of PDGF and PCNA expression.