磁共振灌注成像在鼠肝纤维化、肝硬化病理分级中的价值

目的:分析大鼠肝纤维化、肝硬化磁共振灌注成像(MR-PWI)参数特征,评价PWI对肝纤维化、肝硬化病理分级的价值.方法:清洁级SD大鼠100只,体重约150～200 g,雄性.将大鼠随机分为2组,对照组(n=16),实验组(n=84);实验组腹腔定点注射硫代乙酰胺(TAA),对照组同期腹腔注射相同剂量生理盐水.建模成功后实验组和对照组分批次进行肝脏常规磁共振扫描及PWI扫描.依据病理结果将肝脏损伤分为5组:肝纤维化0期(对照组)、肝纤维化Ⅰ期(S1),肝纤维化Ⅱ期(S2),肝纤维化Ⅲ期(S3),肝硬化结节期(S4),然后分析灌注参数与病理分级的相关性.结果:共69只大鼠(实验组57只,对照组12只)成功获取PWI图像并进行灌注参数测量,成功率69％(69/100).各期门静脉诸灌注参数之间差异有统计学意义(P＜0.05),各期肝实质诸灌注参数之间差异有统计学意义(P＜0.001).门静脉TTP及WOR与肝纤维化分级之间存在相关性(r=0.420、-0.464,P＜0.001).肝实质TTP及WIR与肝纤维化分级之间存在相关性(r=0.424、-0.488,P＜0.001).结论:MR-PWI参数与大鼠肝纤维化、肝硬化的分级有一定相关性.MR-PWI对评价肝纤维化分期具有一定的临床价值.

Value of MR-perfusion weighted imaging in the pathology grading of hepatic fibrosis and cirrhosis of rat

Objective:To analyze the characteristics of MR-perfusion weighted imaging (MR-PWI) of hepatic fibrosis and cirrhosis of rat,and to assess the value of PWI in the pathology grading of liver fibrosis and cirrhosis.Methods:100 clean SD rats,weighting ranged from 150 ～ 180g,male,were randomly divided into two groups:experimental group (n=84) and control group (n=16).Thioacetamide (TAA) was injected into the peritoneal cavity of the experimental group,and the same dose of normal saline was injected into the control group in the same way.Routine MR scanning and MR-PWI were applied afterwards.According to pathology results,liver damage was divided into 5 grades:fibrosis stage 0 (control group),Ⅰ (S1),Ⅱ (S2),Ⅲ (S3) and existence of cirrhosis nodule (S4).The correlation of perfusion parameters and pathology grading was analyzed.Results:PWI images were successfully obtained from 69 rats (69％;control group,n =12;experimental group n=57).There were significant differences among all groups of perfusion parameters of portal vein (P＜0.05),as well as among all groups of perfusion parameters of liver parenchyma (P＜0.001).TTP and WOR of portal vein were correlated with pathology grading of hepatic fibrosis and cirrhosis (r=0.420、-0.464,P＜0.001).TTP and WOR of liver parenchyma were also correlated with pathological grading of hepatic fibrosis and cirrhosis (r=0.424、-0.488,P＜0.001).Conclusion:Correlation was existed between MR-PWI parameters and pathology grading of hepatic fibrosis and cirrhosis of rats.MR-PWI provides clinical value for the grading of hepatic fibrosis.