不同产地温莪术遗传多样性及亲缘关系的ISSR分析＊

目的 采用简单序列重复区间（ISSR）分子标记技术分析不同产地温莪术的遗传多样性和亲缘关系。方法 以3个产地8个温莪术居群的42个样品为材料,从20条ISSR引物中筛选出6条进行PCR扩增,扩增产物经琼脂糖凝胶电泳检测后,利用PopGene 32软件对不同居群ISSR标记结果进行多态性分析,通过UPGMA方法聚类并构建亲缘关系树。结果 6条引物共扩增出 42 个多态性位点,居群水平多态位点平均百分率为29.17%。居群间遗传分化系数Gst为0.3886,居群间每代个体的基因流系数（Nm）为 0.7868。8个居群遗传一致性范围为 0.8481-0.9736,遗传距离范围为 0.0268-0.1647。结论 供试8个温莪术居群之间的遗传多样性水平偏低,遗传变异较少,基因流动性水平较低。本研究的开展为温莪术的引种栽培及育种提供了一定的分子生物学依据。

Genetic Diversity and Phylogenetic Relationship of Curcuma Wenyujin from Different Habitats by ISSR

Objective To analyze the genetic diversity and phylogenetic relationship of Curcuma wenyujin Y.H.Chen et C.Ling from different habitats with ISSR markers.Methods Totally 42 samples from 8 populations of 3 habitats were collected as materials. 6 primers screened from 20 ISSR primers were used for PCR amplification and the amplification products were detected by agarose gel electrophoresis. Popgene32 software was used for polymorphic analysis of different populations and UPGMA method was applied for cluster analysis and construction of phylogenetic tree.Results Totally 42 polymorphic loci were obtained from 6 primers. Percentage of polymorphic loci at population level was 29.17%. The coefficient of gene differentiation (Gst) among populations was 0.3886 and gene flow coefficient (Nm) of individuals was 0.7868. Genetic similarity coefficient of 8 populations was 0.8481-0.9736 and the genetic distances was 0.0268-0.1647.Conclusion Genetic diversity of C. wenyujin from different populations is relatively low. Difference of individuals from different habitats is small at molecular level. The present study provides molecular evidence for introduction, cultivation and breeding of C. wenyujin.