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| 汉防己甲素对体外人眼Tenon囊成纤维细胞的抑制效应 | |
| 引用本文： | 杨柳,李岱,吴开力.汉防己甲素对体外人眼Tenon囊成纤维细胞的抑制效应[J].国际眼科杂志,2009,9(4):653-655. |
| 作者姓名： | 杨柳 李岱 吴开力 |
| 作者单位： | 1. 首都医科大学附属北京天坛医院眼科,中国北京市,100050 2. 湖北省咸宁学院,中国湖北省咸宁市,437100 3. 中山大学中山眼科中心,中国广东省广州市,510060 |
| 基金项目： | 中国国家自然科学基金资助项目，中国广东省自然科学基金，中国湖北省卫生厅基金，中国湖北省咸宁学院基金资助项目 |
| 摘 要： | 目的:研究汉防己甲素(tetrandrine,Tet)对人眼Tenon囊成纤维细胞(Tenon’s capsule fibroblasts,TCFs)的抑制效应。方法:用组织块和混合消化液培养法体外培养人眼Tenon囊成纤维细胞,并对培养的传代细胞进行形态学的鉴定。采用MTT法检测不同浓度的汉防己甲素(10-4,10-5,10-6,10-7mol/L)及0.2g/L丝裂霉素(MMC)对人眼Tenon囊成纤维细胞的抑制作用。结果:人眼Tenon成纤维细胞体外生长良好,经光镜和免疫组化观察证明该细胞为成纤维细胞。MTT法证实,随着Tet药物浓度的增大,TCFs增生活性降低,对应的TCFs抑制率升高。结论:Tet对人眼Tenon囊成纤维细胞的增殖具有明显的抑制作用。 |
| 关 键 词： | 汉防己甲素 成纤维细胞 Tenon囊 MTT |
Inhibitory effect of tetrandrine on the cultured human Tenon's capsule fibroblasts |
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| Liu Yang,Dai Li,Kai-Li Wu.Inhibitory effect of tetrandrine on the cultured human Tenon''s capsule fibroblasts[J].International Journal of Ophthalmology,2009,9(4):653-655. | |
| Authors: | Liu Yang Dai Li Kai-Li Wu |
| Institution: | Liu Yang 1,Dai Li2,Kai-Li Wu31Department of Ophthalmology,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China,2 Xianning College of Hubei Province,Xianning 437100,Hubei Province,3State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,Guangdong Province |
| Abstract: | AIM:To study the inhibitory effect of tetrandrine (Tet) on cultured human fibroblasts from Tenon’s capsule fibroblasts (TCFs). ·METHODS: Human Tenon’s capsule fibroblasts were cultured by organize block and mixed with digestive juice cultivate in vitro. And identified the cultured cell morphology. MTT assay using different concentrations of tetrandrine (10-4, 10-5, 10-6, 10-7mol/L) and 0.2g/L mito-mycin C (MMC) on the person eye Tenon’s capsule fibroblasts in vivo.·RESULTS: The cultured cells grew well in vitro. HE staining and immunohistochemistry study demonstrated fibroblasts. Proliferation activity of TCFs was inhibited with dose-dependent of Tet.·CONCLUSION: Tet could obviously inhibit the prolifera-tion of TCFs in vitro. |
| Keywords: | MTT |
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