| 陡脉冲电场对荷瘤BALB/c小鼠癌细胞杀伤效应的实验研究 |
| |
| 引用本文： | 姚陈果,孙才新,米彦,熊兰,胡娅,胡丽娜.陡脉冲电场对荷瘤BALB/c小鼠癌细胞杀伤效应的实验研究[J].生物医学工程学杂志,2005,22(5):896-900. |
| |
| 作者姓名： | 姚陈果 孙才新 米彦 熊兰 胡娅 胡丽娜 |
| |
| 作者单位： | 重庆大学高电压与电工新技术教育部重点实验室 重庆400044
(姚陈果，孙才新，米彦，熊兰)，重庆医科大学附属第二医院 重庆400010
(胡娅)，重庆医科大学附属第二医院 重庆400010(胡丽娜) |
| |
| 基金项目： | 国家自然科学基金资助项目(50077027) |
| |
| 摘 要： | 观测陡脉冲电场对荷瘤BALB/c小鼠癌细胞的杀伤效应。处理后的癌细胞在光镜下表现为细胞核固缩、核碎裂、核溶解;在电镜下表现为异染色质增加,边集,呈团块样改变,细胞质肿胀,脂滴数量增加,质膜破裂;核固缩,碎裂,线粒体肿胀。实验揭示了陡脉冲电场能有效地杀伤癌细胞,明显抑制了荷瘤小鼠恶性肿瘤的生长、增殖,有着良好的应用前景。
|
| 关 键 词： | 陡脉冲电场 癌细胞 不可逆性电击穿 杀伤效应 |
| 收稿时间： | 2003-07-30 |
| 修稿时间： | 2003-07-302003-12-22 |
Experimental Study of the Lethal Effects of Steep Pulsed Electric Field on Cancer Cells in BALB/c Mice |
| |
| Yao Chenguo, Sun Caixin, Mi Yan, Xiong Lan, Hu Ya, Hu Lina.Experimental Study of the Lethal Effects of Steep Pulsed Electric Field on Cancer Cells in BALB/c Mice[J].Journal of Biomedical Engineering,2005,22(5):896-900. |
| |
| Authors: | Yao Chenguo Sun Caixin Mi Yan Xiong Lan Hu Ya Hu Lina |
| |
| Institution: | 1 .Key Lab of High Voltage Engineering and Electrical New Technology, Ministry of Education. Chongqing University, Chongqing 400044, China;2.Second Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400010, China |
| |
| Abstract: | This study was conducted to observe the lethal effects of steep pulsed electric field(SPEF) on the cancerous squamous cells in BALB/c mice.Female mice were inoculated with the solution of cervical cancer cells line.The tumor-bearing mice of the experiment group were exposed to SPEF. Then, the histomorphological changes were examined and compared between experiment group and control group. The histological and antitumor assay showed that SPEF could selectively injure tumor cells and inhibit cell proliferation. After treatment, pyknosis, karyoclasis and karyolysis of cancer cells were observed under light and electron mircroscope. More serious changes appeared some days later. These results indicate that SPEF have lethal effects on cervical cancer. It may prolong the survival period of tumor-bearing mice. |
| |
| Keywords: | Steep pulsed electric field(SPEF) Cancer cell Irreversible electrical breakdown (IREB) Lethal effects |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
