内源性降钙素基因相关肽在整体大鼠心肌缺血预适应中的作用

为确定预适应缺血时释放的内源性降钙素基因相关肽是否在整体大鼠心肌缺血预适应中起重要作用，分别用放射免疫法测定缺血预适应、降钙素基因相关肽多克隆抗体及对照组的血浆降钙素基因相关肽浓度。并用硝基四唑氮兰染色判定梗死区面积，以坏死区占危险区的百分数表示。结果表明，缺血预适应组第一次和第三次缺血末的血浆降钙素基因相关肽浓度均较对照组显著增高（Ｐ〈０．０１），以后者增加更为明显。缺血预适应能明显抑制缺血／现

The Roles of Endogenous Calcitonin Gene Related Peptide on Myocardial Ischemic Preconditioning in Intact Rat

Aim To investigate whether the release of endogenous calcitonin gene related peptide (CGRP) during preconditioning ischemic insult played an important role in myocardial ischemic preconditioning (IPC) in the intact rat model. Methods Plasma CGRP concentration at the end of first or third ischemic insult was determined with radioimmunoassay. Infarct size as a percentage of the area at risk was determined with nitro blue tetrazolium. Results Mean plasma CGRP levels at the end of first and third ischemic insult were markedly increased in the IPC compared with control(P< 0.01), and it was markedly higher at the end of third ischemia than first in the IPC group (P<0.05). IPC markedly reduced the incidences of ventricular arrhythmias during 30 min ischemia and 2 h reperfusion, which were markedly attenuated by pretreatment with CGRP PcAb. There was a marked reduction infarct size in IPC group (P<0.01) which was markedly attenuated by pretreatment with CGRP PcAb (P<0.01). Conclusion Calcitonin gene related peptide plays an important role in myocardial ischemic preconditioning.