| 硫酸多糖电泳方法的研究 |
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| 引用本文： | 吕志华,赵峡,于广利,王远红,徐家敏.硫酸多糖电泳方法的研究[J].中国生化药物杂志,2002,23(1):17-18. |
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| 作者姓名： | 吕志华 赵峡 于广利 王远红 徐家敏 |
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| 作者单位： | 青岛海洋大学,海洋药物与食品研究所,山东,青岛,266003 |
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| 基金项目： | 国家 8 6 3项目资助 ( 819 0 7 0 3) |
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| 摘 要： | 目的研究几种海洋硫酸多糖的电泳行为。方法采用醋酸纤维膜电泳法和聚丙烯酰胺凝胶电泳(PAGE)法 ,染色剂选用爱茜蓝。结果几种海洋硫酸多糖的醋酸纤维膜电泳均呈单一斑点 ,与标准肝素相比 ,几种海洋硫酸多糖的迁移值不同 ;在建立的PAGE电泳条件下肝素获得了很好的分离 ,几种海洋硫酸多糖的PAGE电泳区带均呈连续分布 ,并利用电泳图象扫描软件进行了相对分子量分布分析。结论醋酸纤维膜电泳法可用于海洋硫酸多糖的定性鉴别 ,PAGE法可用于海洋硫酸多糖相对分子量分布的测定
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| 关 键 词： | 海洋硫酸多糖 电泳 相对分子量分布 |
| 文章编号： | 1005-1678(2002)01-0017-02 |
| 修稿时间： | 2001年4月2日 |
Study on electrophoresis of sulfate polysaccharides |
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| LU Zhi hua,ZHAO Xia,YU Guang li,WANG Yuan hong,XU Jia min.Study on electrophoresis of sulfate polysaccharides[J].Chinese Journal of Biochemical Pharmaceutics,2002,23(1):17-18. |
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| Authors: | LU Zhi hua ZHAO Xia YU Guang li WANG Yuan hong XU Jia min |
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| Abstract: | PurposeThe aim is to study the electrophoresis of sevral marine sulfate polysacchrides. MethodsCellulose acetate membrane electrophoresis(CAME) and polyacrylamide gel electrophoresis(PAGE), were used. The membrane and gel were stained by Alcian blue. ResultsEvery marine sulfate polysacchride had one spot in electrophorogram of CAME and had different migration ratios compared with standard heparin. A good resolution of standard heparin in PAGE was obtained.The zones of marine sulfate polysacchrides were continuously distributed on the gel. The molecular weight distribution was analysed by scanning diagram software.ConclusionCAME could be used as a qualitative differentiation method of marine sulfate polysacchrides, and PAGE could be used to analyse the molecular weight distribution of marine sulfate polysacchrides. |
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| Keywords: | marine sulfate polysaccharide electrophoresis molecular weight distribution |
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