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| 酵母蛋白质组氨酸激酶在昆虫细胞中的表达 | |
| 引用本文： | 王丽颖 杨贵贞. 酵母蛋白质组氨酸激酶在昆虫细胞中的表达[J]. 中国免疫学杂志, 1997, 13(5): 259-261 |
| 作者姓名： | 王丽颖 杨贵贞 |
| 作者单位： | 白求恩医科大学基础医学院 |
| 摘 要： | 以ＤＮＡ聚合酶链式反应从酵母基因组ＤＮＡ中钓取编码酵母组氨酸激酶活性区的ＤＮＡ，将此ＤＮＡ克隆入Ｂａｃｕｌｏｖｉｒｕｓ转递质粒。然后用Ｂａｃｕｌｏ Ｇｏｌｄ ＤＮＡ与重组质粒ｐＶＬ１３９３共转染昆虫细胞（ＳＦ９），在感染了重组Ｂａｃｕｌｏｖｉｒｕｓ的ＳＦ９细胞中表达了ＹＨＫ活性蛋白，该蛋白在体外组氨酸激酶试验中表现出自身组氨酸残基磷酸化的活性。 |
| 关 键 词： | 酵母 蛋白激酶 组氨酸激酶 昆虫 杆状病毒 表达 |
EXPRESSION OF YEAST HISTIDINE KINASE IN INSECT CELLS |
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| Wang Liying,Yang Guizhen. EXPRESSION OF YEAST HISTIDINE KINASE IN INSECT CELLS[J]. Chinese Journal of Immunology, 1997, 13(5): 259-261 | |
| Authors: | Wang Liying Yang Guizhen |
| Affiliation: | Wang Liying,Yang Guizhen.The School of Basic Medical Sciences,Norman Bethune University of Medical Sciences,Changchun 130021 |
| Abstract: | DNA of yeast histidine kinase(YHK) domain from yeast genome isolated by using DNA polymerase chain reaction was cloned into Baculovirus transfer plasmid pVL1393.Recombinant Baculovirus was isolated from SF9 insect cells co transfected with Baculo Gold DNA and recombinant plasmid pVL1393.Expressed recombinant protein isolated from SF9 cells infected with recombinant Baculovirus was demonstrated showing an auto histidine kinase activity in an in vitro assay. |
| Keywords: | Yeast histidine kinase Baculovirus Expression |
| 本文献已被 CNKI 维普 等数据库收录！ | |