MMP-9及其抑制剂TIMP-1在非小细胞肺癌中的表达及其与病理特征的相关性研究

目的研究基质金属蛋白酶-9（MMP-9）和组织基质金属蛋白酶抑制剂-1（TIMP-1）在人非小细胞肺癌（NSCLC）组织中的表达及其与肺癌分期、分化程度和淋巴结转移等病理特征的相关性。方法通过免疫组织化学方法检测非小细胞肺癌组织和癌周组织中MMP-9和TIMP-1的蛋白表达，进行统计学处理。结果MMP-9和TIMP-1在各种不同病理类型的NSCLC组织中有不同程度的表达，在肺癌组织中的表达强于癌周肺组织。MMP-9和TIMP-1在腺癌中的表达强于鳞癌，在不同分化程度的肺癌组织中的表达没有统计学差异，在合并淋巴结转移的肺癌组织中表达较高。结论MMP-9及其特异性抑制剂TIMP-1参与了NSCLC的发生发展过程，MMP-9／TIMP-1表达失衡是NSCLC发生发展的关键。

Expression of MMP-9 and TIMP-1 in Non-Small-Cell Lung Cancer and its Relationship with Clinical Pathological Features

Objective To study the expression of metalloproteinase 9 and tissue inhibitors of metalloproteinase 1 in Non-smallcell lung cancer（NSCLC）, and its relationship with clinical pathological features. Methods The expression of MMP-9 and TIMP-1 in NSCLC and para-cancer pulmonary tissue were tested by immunohistochemical stain. Results The positive expression of MMP-9 and TIMP-1 in specimen was yellow,in cellular plasma. MMP-9 and TIMP-1 express in all types of tissue, strongest in adenocarcinoma, lower in squamous carcinoma, lowest in para-cancer pulmonary tissue. There was no significantly different between well-moderately differented NLCSC and poorly differented NLCSC. There was significantly different between pulmonary tissue with and without lymph node transfer. Conclusion MMP-9 and TIMP-1 may take part in the progress of NSCLC. The inbalaneed expression of MMP-9/TIMP-1 protein is the key enzyme of NSCLC invasion.