木犀草素对膀胱肿瘤T24细胞的影响

目的研究木犀草素对膀胱肿瘤T24细胞体外增殖的抑制和诱导凋亡作用。方法不同浓度木犀草素作用于指数生长期膀胱肿瘤T24细胞后应用MTS比色法检测细胞增殖的抑制作用。通过DNA凝胶电泳检测细胞的凋亡情况。结果浓度为0.01～0.04mg/mL的木犀草素对体外培养的膀胱肿瘤T24细胞有明显的抑制作用。DNA凝胶电泳结果显示:空白对照组没有出现DNA"梯带";其他4组(除木犀草素0.004mg/mL组)均出现不同程度的DNA"梯带",显示出细胞凋亡的特征。结论木犀草素在浓度为0.01～0.04mg/mL时对膀胱肿瘤T24细胞有通过诱导细胞凋亡的方式抑制细胞生长的作用,可能具有治疗膀胱肿瘤的临床价值。

Influence of luteolin on bladder tumor T24 cell

Objective To study the inhibitory effects and inductive apoptosis of luteolin on bladder cancer T24 cell proliferation in vitro.Methods Different concentrations of luteolin were administered in the exponential growth phase of bladder cancer T24 cells,MTS colorimetric assay was used for analysis of cell proliferation inhibition.DNA gel electrophoresis was used to detect the apoptosis.Results The concentration of 0.01-0.04 mg/mL of luteolin inhibited cultured bladder cancer T24 cells significantly.The inhibition rate and concentration are positively correlated.DNA gel electrophoresis showed: blank control group did not show DNA"ladder";the other 4 groups(with the exception of luteolin 0.004 mg/mL group) showed varying degrees of DNA "ladder",indicating apoptotic features.Conclusion Luteolin,at a concentration of 0.04-0.01 mg/mL,has an inhibitory effect on growth of bladder cancer T24 cells by inducing apoptosis,which may be a potential treatment of bladder neoplasm.