Caveolin-1在子宫颈癌及上皮内瘤中的表达及其与VEGF的关系

研究小凹蛋白-1(Caveolin-1)和血管内皮生长因子(VEGF)在正常宫颈、慢性宫颈炎、宫颈上皮内瘤变(CIN)和浸润性宫颈癌组织中的表达,以及与宫颈癌临床病理特征的关系,同时探讨Caveolin-1和VEGF的相关性。方法以正常子宫颈组织30例为对照,应用免疫组织化学SP法检测慢性宫颈炎40例、CINⅠ-Ⅱ级30例、CINⅢ级20例、浸润性宫颈癌40例(TNM分期Ⅰ期21例和Ⅱ期19例)的Caveolin-1、VEGF的表达水平。结果Caveolin-1蛋白在正常宫颈、慢性宫颈炎、CINⅠ-Ⅱ级、CINⅢ级及浸润性宫颈癌组织中的阳性表达率分别为100.00%(30/30)、95.00%(38/40)、83.33%(25/30)、75.00%(15/20)和72.50%(29/40),呈渐进性降低(P<0.05);而VEGF蛋白在正常宫颈、慢性宫颈炎、CINⅠ-Ⅱ级、CINⅢ级及浸润性宫颈癌组织中的阳性表达率分别为10.00%(3/30)、15.00%(6/40)、26.67%(8/30)、45.00%(9/20)和90.00%(36/40),呈渐进性增高(P<0.05)。Caveolin-1、VEGF蛋白在宫颈癌中的表达与年龄、TNM分期无关(P>0.05),与病理分级、浸润深度、区域淋巴结转移有明显关系(P<0.05);Caveolin-1蛋白的表达与肿瘤大小有关系(P<0.05),而VEGF蛋白的表达与肿瘤大小无关(P>0.05)。浸润性宫颈癌及CINⅢ级中Caveolin-1蛋白与VEGF蛋白的表达呈负相关性(列联系数C分别为0.498、0.538,均P<0.05)。结论 Caveolin-1、VEGF是宫颈癌发生中的早期事件,两者可能通过相反的作用机制参与宫颈癌的发生、发展及血管生成过程。

Expression of Caveolin-1 in Cervical Cancer and Intraepithelial Neoplasia and Its Relationship with Vascular Endothelial Growth Factor

Objective To study the expression of caveolin-1 and vascular endothelial growth factor(VEGF) in normal cervix,chronic cervicitis,cervical intraepithelial neoplasia(CIN) and invasive cervical cancer,and to investigate the relationships of Caveolin-1 to VEGF and clinical-pathologic characteristics of Cervical cancer.Methods The expression of Caveolin-1 and VEGF was detected by immunohistochemical staining using streptavidin-biotin peroxidase technology in 30 normal cervical specimens,40 chronic cervicitis,30 CINⅠ-Ⅱ specimens,20 CINⅢ specimens,and 40 invasive cervical cancer specimens(21 stage Ⅰ specimens and 19 stage Ⅱ specimens).Results The positive expression rate of caveolin-1 was 100.00%(30/30),95.00%(38/40),83.33%(25/30),75.00%(15/20) and 72.50%(29/40)in normal cervix,chronic cervicitis,CINⅠ-Ⅱ,CINⅢ and invasive cervical cancer,respectively(P<0.05).The positive expression rate of VEGF was 10.00%(3/30),15.00%(6/40),26.67%(8/30),45.00%(9/20)and 90.00%(36/40)in normal cervix,chronic cervicitis,CINⅠ-Ⅱ,CINⅢ and invasive cervical cancer,respectively(P<0.05).The expression of caveolin-1 and VEGF in cervical carcinoma was not correlated with age and TNM stage(P>0.05),but was significantly correlated with pathological grade,invasion depth and regional lymph node metastasis(P<0.05).The expression of caveolin-1,but not VEGF,was correlated with tumor volume(P<0.05).There was a negative correlation between caveolin-1 protein expression and VEGF protein expression in cervical careinoma and CINⅢ(column connection number C was 0.498 or 0.538,P<0.05).Conclusion The expression of caveolin-1 and VEGF is an early event in cervical tumorigenesis,and caveolin-1 and VEGF may exert opposite effects on tumorigenesis,cancer development and angiogenesis.