BALB/c小鼠结肠癌肝转移模型建立

目的建立BALB/c小鼠结肠癌肝转移模型。方法以BALB/c小鼠为对象,随机分为3组,分别经腹腔脾脏内（分为保脾组和切脾组）、直肠粘膜内注射小鼠结肠癌CT-26细胞0.2ml（浓度为1×107 ml）,观察小鼠平均生存期、成瘤情况、肝转移率、肝脏转移肿瘤大小和其他脏器的转移情况。结果这3种方法均能建立大肠癌肝转移模型,切脾组小鼠平均生存期为（28±5）d,肝转移率为100%,均有肺、胃、膈肌转移及腹水形成,保脾组小鼠平均生存期为（30±5）d,肝转移率为95%,均有肺、胃、膈肌转移及腹水形成;直肠黏膜内注射组小鼠平均生存期为（20±5）d,肝转移率为30%,无肺、胃、膈肌转移及腹水形成。结论保脾组和切脾组比直肠黏膜注射组的成瘤率、转移率更高,小鼠平均生存期较长,同时也增加了其他脏器的肿瘤转移率,建模更稳定,观察期能更好地完成各项指标的评价。

Establishment of colonic carcinoma liver metastasis model in BALB/c Mice

Objective To establish the colonic liver metastases model in BALB/c mice.Methods BALB/c mice were randomly divided into 3 groups with injection of 0.2ml CT-26 CRC cells(1×107ml) by intra-abdominal spleen transplanting(spleen removal group and spleen reservation group) and rectum transplanting respectively.The conditions of all the mice were observed,and the survival time and liver metastases rate were recorded.Results The colonic carcinoma liver metastasis model was established by all of the three ways.As for spleen removal group,the incidence rates of liver metastases was 100%,survival time was(28±5)days,there were lung,stomach and diaphragm transferation;as for spleen reservation group,the incidence rates of liver metastases was 95%,survival time was(30±5)days,there were lung,stomach and diaphragm transferation;as for rectum transplanting group,the incidence rates of liver metastases was 30%,survival time was(20±5)days,no lung,stomach,diaphragm metastases.Conclusion Spleen reservation group and spleen removal group has higher rates of liver metastases and longer survival time than rectum transplanting group.The modeling is more stable and better to complete the evaluation of the indicators in the observation period.