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| 卡介苗作为抗肿瘤重组蛋白疫苗佐剂的研究 | |
| 引用本文： | 任淑萍,万敏,丛宪玲,吴秀丽,王莉,卫红飞,王燕媚,于永利,王丽颖.卡介苗作为抗肿瘤重组蛋白疫苗佐剂的研究[J].中国免疫学杂志,2007,23(6):526-530. |
| 作者姓名： | 任淑萍 万敏 丛宪玲 吴秀丽 王莉 卫红飞 王燕媚 于永利 王丽颖 |
| 作者单位： | 1. 吉林大学公共卫生学院毒理学教研室 2. 吉林大学基础医学院分子生物学教研室,长春,130021 3. 吉林大学中日联谊医院皮肤科,长春,130033 4. 吉林大学基础医学院免疫学教研室,长春,130021 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的：探讨卡介苗（BCG）能否增强抗肿瘤疫苗HSP-MUC1的特异性抑瘤作用，从而将BCG开发为肿瘤疫苗的新型佐剂。方法：动物水平上，BCG和HSP-MUC1共免疫小鼠，观察BCG能否增强HSP-MUC1所激发的特异性抑瘤作用。细胞水平上对BCG发挥佐剂作用的机制进行探讨，将BCG和HSP-MUC1共刺激树突状细胞（DC），观察BCG能否协同HSP-MUC1刺激DC表面的CD86分子表达的上调；并对DC培养上清中IL-6、TNF-α的水平进行测定。结果：BCG＋HSP-MUC1组小鼠的肿瘤重量显著地低于HSP-MUC1组（P〈0.05）。细胞学试验显示，BCG能够显著增强HSP-MUC1对DC的激活作用，使DC表面的CD86分子显著上调。BCG＋HSP-MUC1组的DC培养上清中细胞因子IL-6、TNF-α的水平显著地高于HSP-MUC1组（P〈0.05）。结论：BCG能够显著地增强HSP-MUC1的抗肿瘤活性，具有肿瘤疫苗佐剂的良好功效。 |
| 关 键 词： | 卡介苗 树突细胞 细胞毒性T淋巴细胞 抑瘤作用 |
| 文章编号： | 1000-484X(2007)06-0526-05 |
| 修稿时间： | 2006-02-182007-01-23 |
Research on BCG as adjuvant of antitumor recombinant protein vaccine |
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| REN Shu-Ping,WAN Min,CONG Xian-Ling,WU Xiu-Li,WANG Li,WEI Hong-Fei,WANG Yan-Mei,YU Yong-Li,WANG Li-Ying.Research on BCG as adjuvant of antitumor recombinant protein vaccine[J].Chinese Journal of Immunology,2007,23(6):526-530. | |
| Authors: | REN Shu-Ping WAN Min CONG Xian-Ling WU Xiu-Li WANG Li WEI Hong-Fei WANG Yan-Mei YU Yong-Li WANG Li-Ying |
| Institution: | Department of Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China |
| Abstract: | Objective:To explore the effects of BCG on the tumor suppression elicited by HSP-MUC1 and develop an effective adjuvant of HSP-MUC1.Methods:In animal experiments, BCG was injected into the mice together with HSP-MUC1 to test if BCG can strengthen the tumor suppression elicited by HSP-MUC1; in cellular level, the expression enhancement of CD86 molecules on the surface of dendritic cells by BCG were studied.Results:The tumor weight in mice from BCG+HSP-MUC1 group was significantly less than that from HSP-MUC1 group. The expression of CD86 molecules on the surface of dendritic cells was enhanced by BCG.Conclusion:BCG can be used as adjuvant of HSP-MUC1 and enhance the tumor suppression of HSP-MUC1. |
| Keywords: | HSP-MUC1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |