一种基于表面等离子体共振技术的生物小分子检测方法

目的在以表面等离子体共振生物传感器为核心的便携式生物分子在线分析系统的基础上，建立一种小分子检测方法，满足航天员体液在线检测需求。方法建立小分子物质2，4-二硝基苯肼(DNPH)的检测模型。用DNP—BSA制备生物分子膜；采用抑制检测法，得到检测区间及标准曲线；对检测的可重复性和特异性进行了实验。结果1)DNPH检测的线性范围为7．8ng／ml～2μg／ml，其检测下限为2．5ng／ml；2)生物分子膜的制备及传感器的再生方法能满足多样品测定的需求。结论本方法灵敏、快速、简捷地实现了小分子物质检测，且重复性好、可信度高，在航天医学检测中具有广泛的应用前景。

Detection of Small Molecules Based on Surface Plasman Resonance Technology

Objective: To develop a detective method applied in online assaying of astronauts' humours using the portable online bio-molecules analyzer (POBA) based on surface plasmon resonance biosensor. Method: An assay format was developed based on the detection of 2, 4-Dinitrophenyl-hydrazine. The bio-molecule slide was made by DNP-BSA. Range of detection and standard curve were obtained using inhibition assay. Reliability and specificity of the assay were also tested. Result: 1) The linear range of the assay was 7.8 ng/ml-2 micrograms/ml with lower detection limit of 2.5 ng/ml; 2) Preparation of the bio-molecule slide and regeneration of the biosensor ensured detections for many samples. Conclusion: This assay method can be used to detect small molecules sensitively, rapidly and easily. It can be repeated with good reliability, and has a good application in space medicine.