抗鼠伤寒沙门氏菌抗独特型抗体的研制和初步应用

The Preparation, Identification and immunoprotection of Anti-idiotypic Antibodies against STM H and O Antigen

The immunoprotection of anti-idiotypic antibodies against STM (Salmonella Typhimurium) for adult and neonate BALB/c mice was studied. The mice were immunized with different anti-idiotypic antibodies and STM vaccine. Ten days after last immunizing they were challenged with 20 and 10 LD50 living STM respectively. The immunoprotection is expressed by the survival of mice on 3 days after challenge. The results show that the rate of protection was 62.6% and 71.4% with Ab2-O and its' F(ab')2; 12.5% with both Ab2-H and its' F(ab')2; 75% with Ab2-H + Ab2-O and 100% with inactive STM vaccine for adult mice; 80% with Ab2-O, 66.7% with Ab2-O + Ab1-O, 57.1% with Ab1-O and nonprotection with Ab2-H and STM vaccine for neonate mice. It is known the protective antigen of salmonella is o antigen. These antigens are TI-antigen and are difficult to induce the immunoresponse in neonate mice. Whereas the anti-idiotypic antibodies are TD-antigen, so it can induce such immunity in these animals. The antibodies against H and O antigens of STM were detected from both rabbits and mice immunized by Ab2.The results are shown that the anti-idiotypic antibodies which mimic polysacharids antigen might be used as a vaccine for neonates.