白细胞介素基因启动子区C＋33T多态性与诱导痰中IgE水平相关性研究

目的探讨白细胞介素（IL-4）基因启动子区多态性与儿童哮喘的易感性及诱导痰IgE水平的关系。方法用聚合酶链反应/限制片段长度多态性（PCR-RFLP）的方法对110例哮喘患儿及70例非哮喘对照儿童进行了＋33位点多态性分析,用ELISA法测定诱导痰IgE水平。结果哮喘儿童诱导痰平均IgE为78.51IU/ml,非哮喘对照儿童诱导痰平均IgE为14.45IU/ml,差异有统计学意义（P〈0.001）。哮喘患儿与非哮喘儿童IL-4基因启动子C＋33T位点的等位基因频率和基因型频率CC、CT、TT之间差异有统计学意义（P〈0.001）,且哮喘患儿CT和TT之间均与诱导痰IgE升高相关（P〈0.001）。结论 IL-4启动子C＋33位点多态性与儿童哮喘易感性和诱导痰IgE水平相关。

Correlation between C+33T polymorphism in the IL-4 promoter region and induced sputum IgE levels

Objective To investigate the correlation between the polymorphism of IL-4 promoter region and the susceptibility of children with asthma and the induced sputum IgE levels.MethodsC＋33T polymorphism in the IL-4 promoter region was determined by PCR-RFLP to 110 asthmatic children served as experimental group and 70 agematched children without asthma were used as control.The sputum IgE was detected by ELISA.ResultsThe mean induced sputum IgE level in experimental group and control group was 78.51 IU/ml and 14.45 IU/ml,respectively（P0.001）.The allele frequency of ＋33（C/T） site of IL-4 gene promoter region and genotype frequency of CC,CT,TT was significantly different between experimental group and control group（P0.001）.The analysis of ANOVA model suggested a genetic association of C＋33T polyrnorphism in IL-4 promoter region with induced sputum IgE levels（P 0.001）.Conclusion The C＋33T polymorphism in IL-4 promoter region is associated with susceptibility of allergic asthma and induced sputum IgE levels in Chinese children.