白藜芦醇对脂多糖诱导体外小鼠腹腔巨噬细胞活性的影响

目的探讨白藜芦醇(RES)对脂多糖(LPS)诱导体外小鼠腹腔巨噬细胞(PMA)活化的影响。方法制备腹腔巨噬细胞,分为阴性对照组、LPS组和(RES+LPS)Ⅰ~Ⅵ组,培养24 h后,分别进行中性红染色观察细胞活性。应用酶联免疫吸附法和硝酸还原法实验,用酶标仪检测细胞上清液中肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)和超氧阴离子水平。结果对照组细胞活性比较低,LPS诱导后,腹腔巨噬细胞大量活化,细胞上清液中TNF-α、NO、超氧阴离子水平显著增高,加入RES治疗的PMA细胞活性逐渐减弱,并且相应的细胞上清液中TNF-α、NO、超氧阴离子水平明显降低。结论RES可以抑制PMA的细胞活化,从而减少TNF-α、NO和超氧阴离子的过度分泌。

Effect of resveratrol on oxidative activity of murine abdominal peritoneal macro-phage induced by lipopolysaccharide in vitro

Objective To study the effect of resveratrol on oxidative activity of murine peritoneal macrophages induced by lipopolysaccharied stimulated with lipopolysaccharide ( LPS) in vitro. Methods Peritoneal macrophages were prepared and divided into control group,LPS group and ( RES+LPS) Ⅰ~Ⅵ group. After incubation for 24 h, the activity of neutral red stained cell was observed. Enzyme-linked immunosorbent method and nitrate reduction meth-od were used. The tumor necrosis factor-α ( TNF-α) ,nitric oxide ( NO) and superoxide anion levels of cell superna-tants were detected by microplate reader. Results The cell activity in control group was lower. A large number of peri-toneal macrophages were activated after being induced by LPS,and the TNF-α,NO and superoxide anion levels were significantly higher in cell supernatant;the cell activity of PMA was weakened gradually after RES treatment,and the TNF-α,NO,superoxide anion levels decreased significantly. Conclusion RES can inhibit the cell activation of PMA and reduce the excessive secretion of TNF-α,NO and superoxide anion.