KIR2DL4-IgFc融合蛋白基因真核细胞表达载体的克隆及鉴定分析

目的：构建人KIR2DL4－IgFc段融合蛋白基因真核细胞表达载体。方法：用RT－PCR从孕妇蜕膜组织单个核细胞的总mRNA中逆转录扩增KIR2DL4细胞外段cDNA，经Nhe I和BamH I双酶切后，定向插入真核细胞表达载体CD5lnegl中，然后经酶切和测序鉴定。结果：限制性内切酶切酶 切和序列分析表面已成功构建CD5lnegl-KIR2DL4载体，结论：本研究成功构建KIR2DL4－IgFc融合蛋白真核细胞表达载体，为研究KIR2DL4与其配体之间的关系奠定了基础。

Construction and sequencing of the eukaryotic expression vector of human KIR2DI4-IgFc fusion protein gene from maternal decidual mononuclear cells

ve To construct eukaryotic expression vector of human KIR2DL4-IgFc fusion protein gene. Methods Total cell RNA was extracted from maternal decidual mononuclear cells and the cDNA was amplified by RT-PCR; the cDNA fragments were inserted into the eukaryotic expression vector CD51negl and the recombined plasmid was identified by restriction endonucleases digestion and sequencing. Re-sults After restriction endonucleases treatment and sequencing, it was confirmed that the CD51negl-KIR2DL4 had been constructed succes sfully. Conclusion The recombinant eukaryotic expression vector CD51negl-KIR2DLA was successfully constructed.