抗-HIV初筛试验阳性结果分析

目的探讨检测艾滋病病毒抗体(抗-HIV)的初筛试验方法,了解血清中自身抗体对试验的影响。方法采用酶联免疫吸附试验(ELISA)和胶体金快速试纸条对12 880例临床样本进行抗-HIV检测,抗-HIV初筛阳性样本按规定送确认实验室用免疫印迹法(WB)确认,同时行自身抗体检测。结果第一次初筛抗-HIV阳性15例,第二次双孔复检,同种试剂阳性者15例,另一种试剂阳性9例,WB确认实验9例阳性。15例抗-HIV阳性样本中共检出6例自身抗体阳性:1例RF、ENA阳性,1例抗-dsDNA阳性,2例ANA阳性,1例ENA阳性,1例ANA与SMA阳性。结论抗-HIV初筛试验初、复检双阳性者结果具有较高正确性;血清中自身抗体可引起抗-HIV初筛试验结果假阳性。

Analysis of Positive Results of Primary Screening Test for HIV Antibody

Objective To study the method of primary screening test for anti-HIV and to investigate effect of serum autoantibodies on anti-HIV test. Methods Anti-HIV was detected by ELISA and colloidal gold strips respectively.The positive samples from the primary screening test for anti-HIV must be reconfirmed by the reconfirmation laboratory,and autoantibodies were measured simultaneously. Results The reconfirmed tests of Weston Blot(WB) were performed in 15 serum samples which were positive in the primary screening test for HIV antibody,the results were 9 positive,and 6 negative.The first primary screening test and the second screening test were performed with the same and different reagents,the results were 15 and 9 positive respectively.There were 6 cases autoantibodies found in the 15 anti-HIV positive samples,1 case had RF and ASMA,1 case had anti-dsDNA,2 cases had ANA,and 1 case had ENA. Conclusions When the serum in the first primary and the second screening tests, the positive results are valid.Serum autoantibodies may induce false positive result of anti-HIV test.