L-精氨酸防治胎儿宫内发育迟缓的疗效和机制

目的探讨L-精氨酸(L-Arginine,L-Arg)防治胎儿宫内发育迟缓的疗效,并阐明其治疗机理。方法被动吸烟法建立IUGR孕鼠模型,20只IUGR孕鼠随机分为非干预组和干预组,另选10只孕鼠作为正常对照组。在IUGR孕鼠妊娠的第7~20天给予干预组L-精氨酸。第21天处死孕鼠,测定胎仔体重,鼻-臀长和重量系数,分别测定妊娠末期孕鼠血中NO、ET-1含量和尿液中8-epi-PGF2α的含量。结果干预组胎仔的体重明显增加(P<0.01),孕鼠血清NO水平明显升高(P<0.01),血浆ET-1明显降低(P<0.01),NO/ET-1明显升高(P<0.01),尿液中8-epi-PGF2α的含量明显降低(P<0.01)。结论L-Arg能调节IUGR孕鼠体内NO/ET-1失衡,改善胎盘-子宫-胎儿的血供,减轻其体内的脂质过氧化作用,对IUGR具有较好的干预效果。

Therapeutic effect and mechanism of L-Arginine. on intrauterine growth retardation

Objective To discuss the therapeutic effect and mechanism of L-Arginine on intrauterine growth retardation according to the determination and analysis of serum nitric oxide,plasma endothelin-1 and urine 8-epi-prostaglandin F_(2α).Methods Pregnant rat model with IUGR was established using passive smoking method.Twenty pregnant rats with IUGR were randomized into two groups:Non-intervention group and intervention group.In addition,ten normal pregnant rats were selected into control group.Rats were administered by L-Arginine during 7～20 d pregnancy in intervention group.Pregnant rats were killed on the 21st day of pregnancy.The weight,nose-hip-length and poundera index of these fetal rats were measured.Serum nitric oxide,plasma endothelin-1 and urine 8epi-prostaglandin F_(2α) of pregnant rats were measured respectively.Results In intervention group,fetal weight was obviously increased(P<0.01),Serum nitric oxide and NO/ET-1 in pregnant rats were apparently increased(P<0.01),plasma endothelin-1 and urine 8-epi-prostaglandin F_(2α) in pregnant rats were apparently decreased(P<0.01).Conclusion L-Arginine has good therapeutic effect on IUGR,and it can regulate NO/ET-1 balance,increase placenta-uterus-fetus blood supply,and inhibit lipid peroxidation.