糖尿病大鼠早期视网膜NT-3和GFAP的表达

用链尿佐菌素 (STZ)腹腔注射制作大鼠糖尿病 (DM )模型。分别于模型建立后 1周、2周、1月处死大鼠 ,取眼球做冰冻切片。用免疫组化法定量、半定量分析视网膜神经营养因子 3 (NT 3 )和胶质原纤维酸性蛋白 (GFAP)的含量。结果 :与正常对照组比较 ,DM 1周、2周大鼠视网膜NT 3、GFAP表达无明显改变 ,DM 1月大鼠视网膜含NT 3的神经元数量及神经元内NT 3的含量减少 (P <0 .0 1 ) ,GFAP阳性细胞数量增加 (P <0 .0 5 )。提示 :糖尿病大鼠早期视网膜神经元即已发生损害 ,同时视网膜含NT 3神经元数和神经细胞内NT 3含量均减少 ;视网膜神经细胞的损害可能与NT 3的减少有关。

The Expression of NT-3 and GFAP in Retina in Early Diabetic Rats

The aim was to evaluate the expression of neurotrophin-3 (NT-3) and GFAP in retina of diabetic Wistar rats. Adult male Wistar rats were injected intraperitoneally with streptozotocin (STZ) to create diabetes mellitus. In one week, Two weeks and one month after the model was created, the Wistar rats were killed, and the retina was removed for immunhistochemical expression of NT-3 and GFAP in the retina of diabetic rats and normal rats. Compared with control, there was no change in the expression of NT-3 and GFAP of the Wistar rat retina in the groups with diabetes for one and two weeks; in one month group with diabetes, the expression of NT-3 was obviously reduced in the neuroretina and the expression of GFAP obviously increased in the retina. In the early stage of diabetes mellitus for Wistar rats, the retinal nerve cells have had lesion meanwhile the expression of NT-3 is reduced in the retinal nerve cells. The reduce of NT-3 in retina could be responsible for the lesion of retinal nerve cells.