| PEA3和GDNF在小鼠纹状体的表达 |
| |
| 引用本文： | 景玉萍,沈文英,郑建国,梅盛平,宋本才. PEA3和GDNF在小鼠纹状体的表达[J]. 武汉大学学报(医学版), 2009, 30(4): 441-443,后插二 |
| |
| 作者姓名： | 景玉萍 沈文英 郑建国 梅盛平 宋本才 |
| |
| 作者单位： | 湖北职业技术学院医学院解剖学教研室,湖北,孝感,432000;华中科技大学同济医院解剖学教研室,湖北,武汉,430030 |
| |
| 摘 要： | 目的:观察多瘤促活化因子(PEA3)和胶质细胞源性神经生长因子(GDNF)蛋白在出生后小鼠纹状体神经纤维中的表达变化及相关性,为进一步探讨多巴胺能神经元疾病的治疗方法提供实验依据。方法:分别取正常鼠龄为1,5,10,20,30,40 d昆明小鼠各5只,断头处死,迅速取出大脑,冠状面切取纹状体所在部位1 cm厚的组织块。常规固定、脱水、石蜡包埋,连续切片,免疫组织化学SABC方法,DAB染色。结果:本实验观察到PEA3免疫阳性反应纤维数目在出生后1,5,10,20 d持续上升,在30,40 d下降并保持在一定水平,GDNF蛋白免疫反应阳性纤维数目在出生后1,5,10,20 d保持在一定水平,在30,40 d下降,各组间的表达差异具有统计学意义(P<0.05)。结论:出生后,GDNF在纹状体多巴胺能神经纤维发育过程中发挥作用,并且可能以PEA3作为其下游作用靶。这种作用方式在第20天以前可能是GDNF作用于纹状体神经纤维发育过程的主要途径。
|
| 关 键 词： | PEA3 GDNF 纹状体 神经发育 |
Expression of Polymoma Virus Enhancer-3 and Glial Cell Line-Derived Neurotrophic Factor in Striatum of Mice |
| |
| JING Yuping,SHEN Wenying,ZHENG Jianguo,MEI Shengping,SONG Bencai. Expression of Polymoma Virus Enhancer-3 and Glial Cell Line-Derived Neurotrophic Factor in Striatum of Mice[J]. Medical Journal of Wuhan University, 2009, 30(4): 441-443,后插二 |
| |
| Authors: | JING Yuping SHEN Wenying ZHENG Jianguo MEI Shengping SONG Bencai |
| |
| Affiliation: | JING Yuping1,SHEN Wenying1,ZHENG Jianguo1,MEI Shengping1,SONG Bencai21Dept.of Anatomy,Medical College of Hubei Vocational-Technical College,Xiaogan 432000,China2Dept.of Anatomy,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China |
| |
| Abstract: | Objective: To investigate the expression of polymoma virus enhancer-3(PEA3) and glial cell line-derived neurotrophic factor(GDNF) in striatum of new-born mice.Methods: Kunming mice at age of 1,5,10,20,30,and 40 days were sacrificed to harvest cerebrum for pathological observation.Immunohistochemistry using the method of SABC was adopted to study the expression of PEA3 and GDNF.Results: The numbers of PEA3 positive fibers increased continuously on 1,5,10 and 20 days after born,then decreased to a certain lev... |
| |
| Keywords: | Polymoma Virus Enhancer-3 Glial Cell Line-Derived Neurotrophic Factor Striatum Neural Development |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
