| 聚合酶链反应检测多发性骨髓瘤骨髓细胞IgH基因重排 |
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| 引用本文： | 吕鸣 余润泉. 聚合酶链反应检测多发性骨髓瘤骨髓细胞IgH基因重排[J]. 中国免疫学杂志, 1996, 12(2): 118-120 |
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| 作者姓名： | 吕鸣 余润泉 |
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| 作者单位： | 上海长征医院全军临床免疫中心，上海长征医院血液科 |
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| 摘 要： | 从常规保存的骨髓涂片抽提ＤＮＡ为模板，应用聚合酶链反应（ＰＣＲ）技术检测多发性骨髓瘤（ＭＭ）与反应性浆细胞增多症（ＲＰ）ＩｇＨ基因重排方式。结果３６例ＭＭ中有２９例呈单克隆ＩｇＨ基因重排，８例ＲＰ均呈多克隆重排。表明该方法有助于鉴别ＭＭ与ＲＰ，结合临床其他资料，可作为临床上ＭＭ早期诊断及不典型ＭＭ诊断的手段，具有临床应用价值。
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| 关 键 词： | 多发性骨髓瘤 RP 基因重排 聚合酶链反应 |
DETECTION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT IN MULTIPLE MYELOMA BY POLYMERASE CHAIN REACTON GENE AMPLIFICATION |
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| Lu Ming, Kong Xiantao,Yu Runquan et al.. DETECTION OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT IN MULTIPLE MYELOMA BY POLYMERASE CHAIN REACTON GENE AMPLIFICATION[J]. Chinese Journal of Immunology, 1996, 12(2): 118-120 |
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| Authors: | Lu Ming Kong Xiantao Yu Runquan et al. |
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| Abstract: | Using the DNA extracted from bone marrow smears which have been stored routinely as a template, polymerase chain reaction (PCR) was utilized to detect clonal immunoglobulin heavy chain(IgH)gene rearrangements in multiple myeloma(MM) and reactive plasmacytosis(RP). ClonaL IgH gene rearrangement was detected in 29 of the 36 cases of MM, but not in any of the 8 cases of RP, The results showed: the method was useful in differentiating MM and RP. |
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| Keywords: | Multiple myeloma Reactive plasmacytosis Gene rearrangemen Polymerase chain reaction |
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