被动吸烟对鼠牙槽骨钙、磷含量及碱性磷酸酶活性的影响

目的 分析被动吸烟鼠牙槽骨钙、磷含量和碱性磷酸酶（AKP）的活性。方法 40只6周龄健康雄性Wistar鼠被随机等量分为实验组和对照组,每组再被随机等量分为15、30、45和60 d 4个亚组,烟熏法建立被动吸烟的大鼠模型,浓酸消化法提取牙槽骨内钙和磷,光谱分析法和改良Reddi法分别测定钙、磷含量和AKP的活性,行析因设计的方差分析。结果 与各对照组牙槽骨相比,被动吸烟鼠的钙、磷含量在15 d组略有升高,30 d组略有下降,差异无统计学意义（P〉0.05）,45、60 d组显著下降（P〈0.01）;AKP活性在15、30 d组略升高,45、60 d组略下降,差异无统计学意义（P〉0.05）。结论 本实验周期内,鼠牙槽骨钙、磷含量和AKP活性在被动吸烟初期略有升高,随被动吸烟时间的延长,钙、磷含量呈时间依赖性减少,AKP活性也呈时间依赖性减少的趋势。

Effects of passive smoking on the levels of calcium,phosphorus, and alkaline phosphatase activity in rat alveolar bone

Objective The effects of passive smoking on the levels of calcium, phosphorus, and alkaline phosphatase （AKP） activity in rat alveolar bone were analyzed. Methods Forty healthy 6-week-old male Wistar rats were randomly assigned to experimental and control groups. The experimental rats were further randomized into four subgroups, namely, 15-, 30-, 45-, and 60-day groups, by smoke-exposed function. Concentrated acid digestion method for abstracting bone calcium and phosphorus, spectral analysis, and improved Reddi method were used to examine the levels of calcium, phosphorus, and AKP activity. Measured data were analyzed using ANOVA by factorial design. Results Alveolar bone calcium and phosphorus contents were higher in the 15-day but lower in the 30-day group compared with the other groups; however, no significant changes were found（P〉0.05）. By contrast, significantly lower alveolar bone calcium and phosphorus contents were obtained in the 45- and 60-day groups than those in controls（P〈0.01）. AKP activities of the alveolar bone were slightly higher in the 15- and 30-day groups but lower in the 45- and 60-day than controls; however, no significant changes were found（P〉0.05）. Conclusion Smoke exposure could initially enhance the levels of calcium, phosphorus, and AKP activity in the alveolar bone. However, prolonged passive smoking reduced these three parameters.