改良压碎逸蚴法检测感染性钉螺

目的评价改良压碎逸蚴法检测感染性钉螺的检出率和工效。方法采用双盲对照实验，以压碎法作为金标准，比较改良压碎逸蚴法及压碎法的感染性钉螺检出率，并对尾蚴数进行定量。在现场应用中比较改良压碎逸蚴法、压碎法和逸蚴法的工效。结果改良压碎逸蚴法检出率为100％，每只感染性钉螺体内含尾蚴数（4778±1157）只，一定容积水样检获的尾蚴数与感染性钉螺数呈正相关。改良压碎逸蚴法的工效是压碎法的18．2倍，是逸蚴法的17．3倍。结论改良压碎逸蚴法能快速检测感染性钉螺，并能对感染性钉螺和尾蚴数定量，适用于大批量分处（段）检测阳性钉螺。

Using modified crushing-cercariae escaping method to detect infected snails

Objective To evaluate the detection rate and the efficiency of the modified crushing-cercariae escaping method.Methods The detection rates of the modified crushing-cercariae escaping method and the crushing methods were compared by using a double-blind control experiment with the latter as a gold standard.meanwhile the number of the cercariae was quantified.The efficiency of the two methods aforementioned and the cereariae escaping method were compared in field.Results The detection rate of the modified crushing-cercariae escaping method was 100%.the average number of cercariae in each infected snail was (4 778±1 157);and the number in certain volume of water sample was positively correlated with the number of infected snails.The efficiency of the modified menthed Was 18.2 times and 17.3 times as high as those of the crushing method and cereariae escaping method,respectively.Conclusions The modified crushing-cercariae escaping method Can detect the infected snails quicky and Can quantify the number of infected snails and cercariae,and is suitable for the detection of infected snails in large number.