| 乳牛血清中人HBV样颗粒性质的初步研究 |
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| 引用本文： | 武建国,方宇.乳牛血清中人HBV样颗粒性质的初步研究[J].中国人兽共患病杂志,1989,5(4):20-21. |
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| 作者姓名： | 武建国 方宇 |
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| 作者单位： | 南京军区福州总医院
(武建国，方宇，喻毅强，李芳秋)，南京军区福州总医院(张太和) |
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| 摘 要： | 对免疫电镜发现的乳牛血清中人HBV样颗粒,进一步作了形态学、血清学研究。此颗粒较人HBV颗粒略小。7份阳性血清中,HBsAg(7/7)、HBeAg(6/7)、HBcAg(6/6)、抗-HBs(1/7)阳性,但PHASR、抗-HBc,抗-HBe、32P-人HBVDNA杂交试验及人HBV DNA多聚酶活性均阴性。与马抗-HBs血清作琼脂糖双向扩散试验时出现1～3条沉淀线,其中一条与HBsAg产生的沉淀线融合。
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| 关 键 词： | 乳牛 HBV样颗粒 免疫电镜 |
Preliminary Investigation of Human HBV-Like Particles in Cows Sera |
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| Wu Jianguo.Preliminary Investigation of Human HBV-Like Particles in Cows Sera[J].Chinese Journal of Zoonoses,1989,5(4):20-21. |
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| Authors: | Wu Jianguo |
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| Abstract: | Human HBV-like Particles were visualized by immune - electron microscopy (IBM) in 7 out of 52 cows sera. The Particles showed three forms, small round, tubular and Dane-like of about 20, 20, 40nm in diameter respectively. In these IEM ( ) sera, the HBsAg (7/7) , HBeAg (6/7) , HBcAg (6/6) and anti-HBs (1/7) showed all Positive results with ELISA, but the PHSAR, anti-HBe, anti-HBe, human HBV 32P - DNA hybridization and human HBV DNA Polymerase (two sera tested only) all were negative. There were 1~3 Precipitation lines between the IEM ( ) cows sera and horse anti-HBs serum, one of which fused with that of human HBsAg. |
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| Keywords: | Cows HBV-like part:cles Immuno-electron microscopy |
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