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| 用于布鲁氏菌病酶联免疫吸附试验的三类抗原比较研究 | |
| 引用本文： | 鲁齐发 王慧军. 用于布鲁氏菌病酶联免疫吸附试验的三类抗原比较研究[J]. 中国人兽共患病杂志, 1989, 5(5): 6-8 |
| 作者姓名： | 鲁齐发 王慧军 |
| 作者单位： | 中国预防医学科学院流行病学微生物学研究所(鲁齐发，杨莲芬)，黑龙江省嫩江农场管理局防疫站(王慧军)，黑龙江省农场总局总医院检验科(韩湘云)，黑龙江省农场总局防疫站(卢德威) |
| 摘 要： | 应用布鲁氏菌超声波破碎抗原、脂多糖抗原和试管凝集反应抗原做ELISA分别对实验性布鲁氏菌感染豚鼠及慢性期布鲁氏菌病患者血清抗体的比较研究,初步结果表明,这三类抗原制剂均可做ELISA,用于布鲁氏菌病机体血清抗体的检测。并且与常规布鲁氏菌病血清学试验相比,不仅特异性较好,而且敏感性更高。在这三类抗原制剂中,以超抗的敏感性为最高,SAT抗原次之,而LPS抗原为最差。 |
| 关 键 词： | 布鲁氏菌病 酶联免疫吸附试验 血清学试验 超声波破碎抗原抗体 |
Comparison of 3 Different Antigeic Preparations for the Detection of Chronic Patients and Guinea-pig Serum Antibodies Against Brucella by ELISA |
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| Lu Qifa. Comparison of 3 Different Antigeic Preparations for the Detection of Chronic Patients and Guinea-pig Serum Antibodies Against Brucella by ELISA[J]. Chinese Journal of Zoonoses, 1989, 5(5): 6-8 | |
| Authors: | Lu Qifa |
| Abstract: | An enzyme-linked immunosorbent assay has been developed for the detection of chrinic pstients and guineapig serum antibodies in Brucellosis against whole bacteria,Br. lipopolysaccharide and ultrasonic-broken antigen. It was observed a high level of antibody activity against the antigen and a low level of antibody activity against LPS. But these different antigen preparation were suitable to a diagnostic antigen in an ELISA for serum antibodies detection of Brucellosis.These results indicate that ELISA is a very sensitive test but its value as a serodiagnostic procedure is dependent upon the choice of antigen used in the assay. |
| Keywords: | Brucellosis ELISA Serological tests Ultrasonic-broken antigen atibody. |
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