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Phagolysosomal pH and Location of Particles in Alveolar Macrophages |
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| Authors: | NYBERG, KRISTINA JOHANSSON, UNO JOHANSSON, ANNE CAMNER, PER |
| Affiliation: | *Section of Lung Medicine, Unit of Environmental Hygiene, Institute of Environmental Medicine, Karolinska Institute S-104 01 Stockholm, Sweden !["{dagger}"]("/math/dagger.gif") Department of Pathology II, University of Link?ping S-581 85 Link?ping, Sweden !["{ddagger}"]("/math/Dagger.gif") The Wenner-Gren Institute, the Arrhenius Laboratories for Natural Sciences, University of Stockholm S-106 91 Stockholm, Sweden Received April 16, 1990; accepted November 28, 1990 |
| Abstract: | Phagolysosomal pH and Location of Particles in Alveolar Macrophages.NYBERG, K., JOHANSSON, U., JOHANSSON, A., AND CAMNER, P. (1991).Fundam. Appl. Toxicol. 16, 393400. Fluorescein-labeledsilica particles (FSP) were instilled into the tracheae of rabbits.Groups of four rabbits were killed after 24 hr, 1 week, 1 month,or 3 months and their lungs were lavaged. Phagolysosomal pHin the alveolar macrophages (AM) was measured using microscopefluorometry with FSP as a probe. Due to the marked decline ofthe fluorescence intensities from the FSP between 1 and 3 monthsafter instillation, it was not possible to measure pH at 3 months,but the values from 24 hr, 1 week, and 1 month were quite similar,with group means of 4.8 and 4.9, respectively. PhagolysosomalpH in AM which phagocytized the FSP in vitro showed values abouthalf a pH unit higher. AM from rabbits lavaged at 1 week hadmore lysosomes in contact with the FSP-containing phagolysosomesand a higher degree of vacuolization between the FSP and thephagolysosomal membrane than AM lavaged at 1 day. The locationof the FSP in the AM appeared to be similar in rabbits lavagedafter 1 week and 3 months. In histologic sections from the lungsthe large majority of the FSP were within cells at all timepoints. |
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