| 高效液相色谱法测定利咽口服液中甘草酸的含量 |
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| 引用本文： | 林方,庄阳辉.高效液相色谱法测定利咽口服液中甘草酸的含量[J].海峡药学,2012,24(7):55-56. |
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| 作者姓名： | 林方 庄阳辉 |
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| 作者单位： | 福建福州市第二医院,福州,350001 |
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| 摘 要： | 目的建立利咽口服液中甘草酸的含量测定方法。方法采用高效液相色谱法,色谱柱为Lichrospher C18柱(4.6×250mm,5μm),流动相为甲醇-0.2mol·L-1醋酸铵溶液-冰醋酸(66∶33∶1.3),检测波长为250nm,流速为1.0mL·min-1,柱温为30℃。结果本法精密度高,稳定性好,甘草酸浓度在10.9172~174.6752μg·mL-1范围内具有良好的线性关系(r=0.9999);平均加样回收率99.1%,RSD为1.14%。结论本方法简便,准确,重现性好,可作为该制剂的含量测定方法。
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| 关 键 词： | 利咽口服液 甘草酸 高效液相色谱法 |
Determination of glycyrrhizic acid in Liyan oral liquid by HPLC |
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| LIN Fang
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ZHUANG Yang-hui.Determination of glycyrrhizic acid in Liyan oral liquid by HPLC[J].Strait Pharmaceutical Journal,2012,24(7):55-56. |
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| Authors: | LIN Fang ZHUANG Yang-hui |
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| Institution: | (Fuzhou Second Hospital, Fuzhou 350007, China) |
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| Abstract: | OBJECTIVE To establish a method for determination of glycyrrhizic acid in Liyan Oral Liquid.METHODS Using a HPLC method with Lichrospher Cls column （4.6 x 250mm, 5μm）, themobile phase was a methanol - 0.2mol. L- 1 ammonium acetate-glacial acetic acid （66 ： 33： 1.3）, the detection wavelength at 250nm, flow rate was 1.0mL. min-l, and column temperature was 3012. RESULTS The precision and stability were fine, The standard curve of glycyrrhizic acid was rectilinear within the range of 10. 9172 - 174. 6752μg. mL- 1, r = 0. 9999. The average recovery of glycyrrhizic acid was 99.1% with RSD of 1.14 %. CONCLUSION The method was simple, accurate and reproducible, and can be used for the determination of glycyrrhizic acid. |
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| Keywords: | Liyan Oral Liquid glycyrrhizic acid HPLC |
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