microRNA靶序列单核苷酸多态性与胃癌易感性关系

目的研究炎性相关基因IL-1F5（interleukin-1 factor 5）的3’非编码区microRNA（miRNA）靶序列单核苷酸多态性（SNP）与胃癌易感性关系。方法采用1∶1频数匹配病例-对照研究对研究对象进行基因分型，应用多因素条件logistic回归及多因素降维法（MDR）分析SNP与胃癌易感性的关系及其与环境因素的交互作用。结果rs2472188位点GC基因型（OR=1.50，95%CI=1.13~1.99）和rs2515401位点CT基因型（OR=1.42，95%CI=1.09~1.86）是胃癌的易感基因；单体型分析显示，单体型1（CCGCA）（OR=2.08，95%CI=1.27~3.40）、单体型4（CTATA）（OR=1.98，95%CI=1.48~2.66）、单体型5（CTATC）（OR=10.13，95%CI=4.43~23.16）可增加胃癌发病风险，而单体型2（CTACA）（OR=0.18，95%CI=0.12~0.28）、单体型3（CTACC）（OR=0.37，95%CI=0.23~0.59）、单体型9（GCGTC）（OR=0.39，95%CI=0.27~0.58）可降低胃癌发病风险；具有rs2472188、rs2515401以及rs2515402组合的人群是胃癌发病风险的高危人群（OR=6.17，95%CI=4.61~8.36）。结论rs2472188位点GC基因型、rs2515401位点CT基因型是胃癌的易感基因型，多位点联合作用对于胃癌的发生可能有协同作用。

Correlation between single nucleotide polymorphism of microRNA target site and gentic susceptibility of gastric cancer

Objective To assess the correlation between single nucleotide polymorphism(SNP)of microRNA(miRNA)target site and the gentic susceptibility of gastric cancer. Methods A 1:1 matched case-control study was adopted in this study.Genotyping was carried out among the subjects.Conditional logistic regression model and multifactor-dimensionality reduction(MDR)method were used to analyze the correlation between SNP of interleukin-1 factor 5(IL-1F5)and genetic susceptibility of gastric cancer.The gene-environment interaction was also analyzed. Results GC genotype in rs2472188 locus(odds ratio [OR]=1.50,95% confidence interval [95%CI]=1.13-1.99)and CT genotype in rs2515401 locus(OR=1.42,95%CI=1.09-1.86)were susceptible genotypes for gastric cancer.Haplotype analysis showed that haplotype 1(CCGCA)(OR=2.08,95%CI=1.27-3.40,haplotype 4(CTATA)(OR=1.98,95%CI=1.48-2.66)and haplotype 5(CTATC)(OR=10.13,95%CI=4.43-23.16)increased the risk of occurrence of gastric cancer,whereas haplotype 2(CTACA)(OR=0.18,95%CI=0.12-0.28),haplotype 3(CTACC)(OR=0.37,95%CI=0.23-0.59)and haplotype 9(GCGTC)(OR=0.39,95%CI=0.27-0.58)decreased the risk. Conclusion Genotypes of GC in rs2472188 locus and CT in rs2515401 locus may be susceptible genotypes for gastric cancer.Combined effects of multigene alleles and multi-locus genotype may have a synergistic effect in the carcinogenesis of gastric cancer.