| 光气致小鼠肺组织细胞凋亡相关基因的表达 |
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| 引用本文： | 李文丽,梁欣,张伟,柏桦,吴昊,海春旭. 光气致小鼠肺组织细胞凋亡相关基因的表达[J]. 癌变．畸变．突变, 2010, 22(6): 436-439,443 |
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| 作者姓名： | 李文丽 梁欣 张伟 柏桦 吴昊 海春旭 |
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| 作者单位： | 第四军医大学军事预防医学系毒理学教研室,陕西,西安,710033 |
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| 基金项目： | 军队十一五指令课题(06Z038) |
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| 摘 要： | 目的：研究光气染毒肺水肿小鼠肺组织Bax、c-Fos、c-Jun、Caspase-3和Fas的表达。方法：10只BALB/c小鼠随机分为对照组和光气染毒组,每组5只。正常对照组以空气为对照,染毒组小鼠给予11.9 mg/L的光气,染毒时间为5 min。染毒后4 h,取小鼠肺脏,用4%的多聚甲醛固定。原位杂交和免疫组化测定肺组织Bax、c-Fos、c-Jun,Caspase-3和Fas的表达。Westen blot测定Fas蛋白的表达。结果：光气染毒组肺水肿小鼠肺组织Bax、c-Fos、c-Jun、Caspase-3和Fas基因的表达较对照组明显增高（P〈0.05）,且Fas蛋白的表达也升高（P〈0.05）。结论：光气可能通过Fas/FasL通路诱导肺上皮细胞发生凋亡。
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| 关 键 词： | 光气 肺水肿 凋亡 氧化应激 |
Expression of apoptosis-related genes in mouse lungs induced by phosgene |
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| LI Wen-li,LIANG Xin,ZHANG Wei,BAI Hua,WU Hao,HAI Chun-xu. Expression of apoptosis-related genes in mouse lungs induced by phosgene[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2010, 22(6): 436-439,443 |
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| Authors: | LI Wen-li LIANG Xin ZHANG Wei BAI Hua WU Hao HAI Chun-xu |
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| Affiliation: | LI Wen-li,LIANG Xin,ZHANG Wei,BAI Hua,WU Hao,HAI Chun-xu (Department of Toxicology,School of Military Preventive Medicine,Fourth Military Medical University,Xi'an 710033,Shaanxi,China) |
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| Abstract: | OBJECTIVE: To study the expressions of Bax,c-Fos,c-Jun,Caspase-3 and Fas in pulmonary edema induced by phosgene in mice.METHODS: 10 BALB/C mice were divided into control group and phosgene group with 5 mice in each.Mice were exposed to air and phosgene for 5 minutes in control and phosgene group,respectively.Dose of phosgene was 11.9 mg/L.After 4 hours' exposure,all mice were immediately anesthetized.Lungs were fixated by 4% citromint.The expressions of Bax,c-Fos,c-Jun,caspase-3 and Fas gene were determined... |
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| Keywords: | phosgene pulmonary edema apoptosis |
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