| 二氧化锗诱导L6成肌细胞株的MyoD基因表达 |
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| 引用本文： | 邱小忠,余磊,张黎声,廖华,秦建强,杨俊,欧阳钧. 二氧化锗诱导L6成肌细胞株的MyoD基因表达[J]. 中国临床解剖学杂志, 2005, 23(1): 14-16 |
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| 作者姓名： | 邱小忠 余磊 张黎声 廖华 秦建强 杨俊 欧阳钧 |
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| 作者单位： | 1. 南方医科大学临床解剖学研究所,广东省组织构建与检测重点实验室,广州,510515
2. 上海中医药大学解剖学教研室,上海,200032 |
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| 基金项目： | 广东省专项课题(A302020401);广东省科技攻关项目(2KM05503S)和广州市科技攻关项目(2002Z1-E0037) |
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| 摘 要： | 目的：探讨成肌细胞的MyoD基因在线粒体肌病发生发展中的作用。方法：采用二氧化锗(GeO2)处理大鼠的成肌细胞系L6，观察细胞形态的变化，利用MTT分析GeO2对成肌细胞的影响，用RT-PCR检测MyoD基因表达。结果：发现GeO2在损伤成肌细胞的同时，能够诱导MyoD基因的表达，表明MyoD基因在线粒体肌病的发生发展中起着重要作用。结论：MyoD基因表达的增强是线粒体肌病中骨骼肌萎缩的一个信号分子，MyoD基因表达有可能成为线粒体肌病检测的参考指标。
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| 关 键 词： | 二氧化锗（GeO2） L6细胞株 MyoD基因 线粒体肌病 |
| 文章编号： | 1001-165X(2005)01-0014-03 |
| 修稿时间： | 2004-05-18 |
Expression of MyoD gene in L6 myoblast cells induced by GeO2 |
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| QIU Xiao-zhong,YU lei,ZHANG Li-sheng,et al.. Expression of MyoD gene in L6 myoblast cells induced by GeO2[J]. Chinese Journal of Clinical Anatomy, 2005, 23(1): 14-16 |
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| Authors: | QIU Xiao-zhong YU lei ZHANG Li-sheng et al. |
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| Affiliation: | QIU Xiao-zhong,YU lei,ZHANG Li-sheng,et al.Department of Anatomy,Key Laboratory of Tissue Construction and examination of Guangdong province,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective: To explore the relationship between the MyoD gene of myoblast cells and the development of mitochondrial myopathy. Methods: The L6 myoblast cells were treated by GeO2. The morphological and molecular changes of cells were described through the MTT and RT-PCR methods respectively and RT-PCR was used to analysis the expression of MyoD gene. Results: The myoblast cells were damaged by GeO2, meanwhile the GeO2 induced the expression of MyoD gene. Conclusions: The enhanced expression of MyoD gene is a molecular symbol for skeletal muscle damages in the mitochondrial myopathy. The MyoD gene maybe a useful diagnostic marker in mitochondrial myopathy diseases. |
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| Keywords: | GeO2 L6 cells MyoD gene mitochondrial myopathy |
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