汉防己甲素联合屈洛昔芬对K562和K562/A02细胞NF-κB蛋白表达的影响

目的 研究汉防己甲素(Tet)和屈洛昔芬(DRL)单独及联合用药后对K562细胞及其耐药细胞株K562/A02的核因子κB(NF-κB)表达的影响,以进一步探讨其逆转多药耐药的作用机制.方法 采用免疫细胞化学及Western blotting法分别检测Tet(1μmol/L)、DRL(5 μmol/L)单独及联合作用于K562和K562/A02细胞后NF-κB核转位水平和胞核NF-κB蛋白表达水平的变化.结果①K562/A02细胞NF-κB核转位水平(65.0±4.2)%]及胞核NF-κB蛋白表达(3.545±0.219)明显高于K562细胞(39.6±0.9)%和2.366±0.141](P＜0.01);②Tet、DRL单独及联合作用6、12 h,对K562细胞NF-κB核转位水平及胞核NF-κB蛋白表达水平无明显影响(P＞0.05);③两药单独及联合作用6、12 h,可明显降低K562/A02细胞NF-κB蛋白核转位水平和胞核NF-κB蛋白表达水平(P＜0.05和P＜0.01),两药联合作用增强(P＜0.05),作用12 h较作用6 h效果更显著(P＜0.05).结论K562/A02细胞的胞核NF-κB蛋白表达水平明显高于K562细胞,NF-κB的活化可能参与了K562/A02细胞耐药的发生;抑制NF-κB活化可能参与了Tet和DRL逆转K562/A02细胞多药耐药过程.

Effect of tetrandrine in combination with droloxifen on the expression of NF-κB protein in K562 and K562/A02 cell lines

Objective To study the effect of tetrandrine (Tet) in combination with droloxifen(DRL)on the expression of nuclear factor kappa B(NF-κB) in K562 and K562/A02 cell lines and its reversal mechanism. Methods The activation of NF-κB in K562 and K562/A02 cell lines and the effect of Tet or DRL alone or in combination on NF-κB protein expression were determined with immunocytochemistry and down-regulate NF-κB protein expression in nuclei of K562/A02 cells. The effect was more significant in commay be involved in the reversal of multidrug resistance in K562/A02 cells by Tet and DRL.