封闭型空间细胞培养板研制与验证

目的为适用于空间特殊实验环境,并在细胞培养实验中更高效地使用试剂、提高细胞培养用品的污染防护能力,提出了一种内部流动路径可控、具备防渗漏、防污染能力的全透明和全封闭细胞培养板。方法基于有限元仿真对培养板核心结构—流路控制挡板进行了优化,以优化后的培养板为核心部件搭建细胞培养回路,并开展了中长周期细胞培养试验。结果最优化的三坝-纵向排列细胞培养板,可有效控制其内部流体的流动路径,进出口流速1 m L/min时流体剪切力仅4.8×10~(-5)Pa,流动死区几乎为0,以该培养板为核心构建的细胞培养回路中培养的细胞可正常生长15 d。结论该细胞培养板,可使腔内新旧细胞培养试剂更换及残留气体排出更彻底,防渗漏防污染能力大大提升,试剂使用率提高,能满足空间实验常用典型细胞系的中长期培养需求,适合于我国目前及将来的空间医学生物学实验应用场合。

Development and Validation of a Closed Cell Culture Plate for Space Applications

Objective In order to make the cell culture plates fit for space experiments,to use reagents more efficiently in cell culture experiments,and to improve the anti-contamination ability of cell culture materials,a fully transparent closed cell culture plate with controlled inner flow path and anti-leakage/anti-contamination capability was proposed.Methods The arrangement of the flow path control bar which is the core structure of the culture plate was optimized based on the finite element simulation (FES).The optimal cell culture plate was fabricated,then the mid-long-term cell culture experiments were carried out in the cell culture loop with the aforementioned cell culture plate as its key component.Results The optimal cell culture plate with three longitudinally arranged bars could form well-controlled flow channels in its chamber.When the flow velocity at inlet and outlet was 1 mL/min,the fluid shear stress in the chamber was only 4.8 × 10-5Pa.And there was almost no flow dead zone.The cells cultured in the cell culture loop with the cell culture plate as its core component could proliferate normally throughout the 15 d experimental period.Conclusion The cell culture plate has the following advantages:the replacement of old and new cell culture reagents and residual gas discharge are more thorough;anti-leakage and anti-contamination capability are significantly improved;and reagent usage rate is increased.It is suitable for the mid-long-term culture of the typical cells used in the space medical biology experiments.