胰蛋白酶法和Triton—X100法去除瓣膜细胞的效果比较

目的比较胰蛋白酶法和Triton—X100法去除异种或同种心脏瓣膜上细胞的效果。方法选取新鲜猪主动脉瓣膜片分别置入0．05％胰酶溶液和0．25％Triton-X100溶液中处理48h，并以未经处理的新鲜瓣膜片作对照，行光镜及电镜观察．并作热皱缩温度、断裂强度和断裂拉伸率测定。结果光镜和电镜下均显示，胰酶法和Triton-X100法均彻底去除了主动脉瓣膜上的细胞成分，但Triton—X100法对瓣膜支架结构的损伤较大。热皱缩温度、断裂强度和断裂拉伸率在新鲜瓣膜组和胰酶组间无显著性差异，但Triton—X100组与新鲜瓣膜组及胰酶组相比均有下降。结论胰酶法去除猪主动脉瓣膜细胞彻底．保持了瓣叶支架结构，优于Triton—X100法。

Effect of trypsin and Triton-X 100 for decellularization of porcine aortic heart valves]

OBJECTIVE: To test two decellularization procedures for their potential of cell removal and mormal matrix preservation. METHODS: Porcine aortic valve leaflets were treated with either 0.05% trypsin or 0.25% Triton-X 100 respertively for 48 h for decellularization and with fresh untreated valve leaflets as control. Two tissue samples from each group were stained with hematoxylin and eosin and observed light-microscopically followed by scanning electron microscopy. Ten valve leaflets in each group were measured for shrinkage temperature, tensile strength/fracture toughnes and percentage elongation. RESULTS: Trypsin and Triton-X 100 all achieved complete decellularization but Triton-X 100 caused stronger structural alterations. No significant difference was identified between untreated and trypsin groups in shrinkage temperature, tensile strength/fracture toughnes and percentage elongation, but Triton-X 100 group showed significant difference from the other two groups. CONCLUSION: Decellularization using trypsin is superior to Triton-X100 in efficiency and matrix preservation.